ArticleNature communications2019
Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Guiding AlphaFold predictions with experimental knowledge to inform dynamics and interactions with VAIRO.Protein science : a publication of the Protein Society · 2026Article
- Cryo-EM structure and polar assembly of the PS2 S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract.Nature communications · 2024Article
- SymProFold: Structural prediction of symmetrical biological assemblies.Nature communications · 2024Article
- The direct inhibitory effects of Lactobacillus acidophilus, a commensal urinary bacterium, on calcium oxalate stone development.Microbiome · 2024Article
- Time-resolved cryogenic electron tomography for the study of transient cellular processes.Molecular biology of the cell · 2024Article
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- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Article
- ThePNAS nexus · 2022Article
- Nested Formation of Calcium Carbonate Polymorphs in a Bacterial Surface Membrane with a Graded Nanoconfinement: An Evolutionary Strategy to Ensure Bacterial Survival.ACS biomaterials science & engineering · 2022Article
- High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.Structure (London, England : 1993) · 2022Article
- Molecular Logic of Prokaryotic Surface Layer Structures.Trends in microbiology · 2021Review
- The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-OxidizingFrontiers in microbiology · 2021Article
- Spatial organization of Clostridium difficile S-layer biogenesis.Scientific reports · 2020Article
- Lipopolysaccharide O-antigens-bacterial glycans made to measure.The Journal of biological chemistry · 2020Review
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Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Many bacteria and most archaea possess a crystalline protein surface layer (S-layer), which surrounds their growing and topologically complicated outer surface. Constructing a macromolecular structure of this scale generally requires localized enzymatic machinery, but a regulatory framework for S-layer assembly has not been identified. By labeling, superresolution imaging, and tracking the S-layer protein (SLP) from C. crescentus, we show that 2D protein self-assembly is sufficient to build and maintain the S-layer in living cells by efficient protein crystal nucleation and growth. We propose a model supported by single-molecule tracking whereby randomly secreted SLP monomers diffuse on the lipopolysaccharide (LPS) outer membrane until incorporated at the edges of growing 2D S-layer crystals. Surface topology creates crystal defects and boundaries, thereby guiding S-layer assembly. Unsupervised assembly poses challenges for therapeutics targeting S-layers. However, protein crystallization as an evolutionary driver rationalizes S-layer diversity and raises the potential for biologically inspired self-assembling macromolecular nanomaterials.
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