Evidence map›Paper›PMID 31227565›Full record

ArticleRNA (New York, N.Y.)2019

Sensitive and quantitative probing of pseudouridine modification in mRNA and long noncoding RNA.

Wen Zhang, Matthew J Eckwahl, Katherine I Zhou, Tao Pan

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 71 citations in OpenAlex.

  1. Ψ-Atlas: An Integrated Atlas for Pseudouridine Epitranscriptome.Genomics, proteomics & bioinformatics · 2026
    Article
  2. Integrating mass spectrometry with Nanopore direct RNA sequencing forbioRxiv : the preprint server for biology · 2026
    Article
  3. Pseudouridylation landscape across 42bioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Functions and therapeutic applications of pseudouridylation.Nature reviews. Molecular cell biology · 2025
    Review
  13. Article
  14. Science advances · 2025
    Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Wen ZhangDepartment of Chemistry, University of Chicago, Chicago, Illinois 60637, USA.
Matthew J EckwahlDepartment of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA.
Katherine I ZhouDepartment of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA.
Tao PanDepartment of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA.
University of Chicago · US

Funding

MEDICAL SCIENTIST TRAININGT32GM007281 · NIGMS · UNIVERSITY OF CHICAGO · PI CLARK, MARCUS RAMSAY · 1985 to 2022
$32.1M
Transcriptome-wide base-resolution sequencing of pseudouridine in human normal and AD brain tissues-CEGS Admin supplement 2020-2021RM1HG008935 · NHGRI · UNIVERSITY OF CHICAGO · PI HE, CHUAN · 2016 to 2025
$24.4M
MULTIDISCIPLINARY CARDIAC SCIENCEST32HL007381 · NHLBI · UNIVERSITY OF CHICAGO · PI JEANNE M DECARA, Yoav Gilad · 1985 to 2026
$12.5M
Role of m6A in HNRNPG protein localization and HNRNPG-regulated alternative splicingF30GM120917 · NIGMS · UNIVERSITY OF CHICAGO · PI ZHOU, KATHERINE ISMEI · 2016 to 2018
$147k
Global profiling of N6-methyladenosine (m6A) with Deam-seq and Circtron-seqF32GM126745 · NIGMS · UNIVERSITY OF CHICAGO · PI ECKWAHL, MATTHEW · 2018 to 2018
$34k
NHGRI NIH HHS RM1 HG008935NHLBI NIH HHS T32 HL007381NIGMS NIH HHS F30 GM120917NIGMS NIH HHS F32 GM126745NIGMS NIH HHS T32 GM007281
6 · The paper itself

Abstract

Pseudouridine (Ψ) is the most abundant RNA modification in cellular RNA present in tRNA/rRNA/snRNA and also in mRNA and long noncoding RNA (lncRNA). Elucidation of Ψ function in mRNA/lncRNA requires mapping and quantitative assessment of its modification fraction at single-base resolution. The most widely used Ψ mapping method for mRNA/lncRNA relies on its reaction with

Indexed as

Cell LineCell Line, TumorHEK293 CellsHeLa CellsHumansMCF-7 CellsPseudouridineReal-Time Polymerase Chain ReactionReverse TranscriptionRNA, Long NoncodingRNA, MessengerRNA, RibosomalRNA, Small NuclearRNA, TransferPseudouridineRNA, Long NoncodingRNA, MessengerRNA, RibosomalRNA, Small NuclearRNA, TransfermRNApseudouridineRT-PCR

Identifiers

PMID31227565
PMCPMC6800517
OpenAlexW2952790249

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.