Evidence map›Paper›PMID 31226941›Full record

ArticleBMC gastroenterology2019

Histopathological features of the proper gastric glands in FVB/N-background mice carrying constitutively-active aryl-hydrocarbon receptor.

Ai Dantsuka, Osamu Ichii, Annika Hanberg, Yaser Hosny Ali Elewa, Saori Otsuka-Kanazawa, Teppei Nakamura, Yasuhiro Kon

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC gastroenterology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Ai DantsukaLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan.
Osamu IchiiLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan.
Annika HanbergInstitute of Environmental Medicine, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
Yaser Hosny Ali ElewaLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan.
Saori Otsuka-KanazawaLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan.
Teppei NakamuraLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan.
Yasuhiro KonLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan, Kita 18, Nishi 9, Kita-ku, Sapporo, 060-0818, Japan. y-ko-n@vetmed.hokudai.ac.jp.
Hokkaido University · JPKarolinska Institutet · SEZagazig University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAryl-hydrocarbon receptor (AhR) is a multiple ligand-activated transcription factor that has important roles in xenobiotic, physiological, or pathological functions. Transgenic mice systemically expressing constitutively-active AhR (CA-AhR) have been created to mimic activated AhR signaling in vivo. However, their detailed histopathological features are unclear. In the present study, we generated CA-AhR-expressing FVB/N mice (FVB-CA-AhR mice) and clarified their phenotypes in detail.

methodsMale and female FVB-CA-AhR and wild-type mice were histopathologically examined from 6 to 33 weeks of age.

resultsAmong the systemic organs, only the stomachs in FVB-CA-AhR mice showed pathological changes including cystic structures beneath the serosa; in addition, stomach weights increased with age. Histopathologically, cystic structures and alcian blue-positive metaplasia were observed in the mucosa of the proper gastric glands, and these two histometric parameters were positively correlated. Furthermore, proliferating cells shifted from the isthmus to the base of the glands, and parietal cells decreased. Age-related histopathological changes were clearer in females than in males. Importantly, in FVB-CA-AhR mice, intramucosal cysts developed as extramucosal cysts beneath the serosa, penetrating the lamina muscularis mucosae and the muscularis propria. Their incidence reached 100% in 28-week-old male mice and 33-week-old female mice. Extramucosal cysts contained alcian blue-, Griffonia simplicifolia lectin II-, or trefoil factor 2-positive cells, suggesting a stomach origin for the cysts and spasmolytic polypeptide-expressing metaplasia-like lesions.

conclusionsDisease onset occurred earlier in FVB-CA-AhR mice than previously reported in C57BL/6-derived CA-AhR mice. Importantly, the histopathological features were partly similar with gastritis cystica profunda in humans and animals. Excessive activation of AhR signaling aggravated abnormalities in the gastric mucosa and were affected by both genetic- and sex-related factors.

Indexed as

Alcian BlueAnimalsBasic Helix-Loop-Helix ProteinsCystsFemaleGastric MucosaIntercellular Signaling Peptides and ProteinsMaleMetaplasiaMiceMice, Inbred C57BLMice, TransgenicPhenotypePlant LectinsReceptors, Aryl HydrocarbonSignal TransductionAhr protein, mouseAlcian BlueBasic Helix-Loop-Helix ProteinsGriffonia simplicifolia lectinsIntercellular Signaling Peptides and ProteinsPlant LectinsReceptors, Aryl Hydrocarbonspasmolytic polypeptideTFF2 protein, mouseTrefoil Factor-2Constitutively-active aryl-hydrocarbon receptorCystFVB/N miceGastric mucosal abnormalityMetaplasia

Identifiers

PMID31226941
PMCPMC6588904
OpenAlexW2949934606

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.