ArticleMicrobial biotechnology2019
Impact of spray-drying on the pili of Lactobacillus rhamnosus GG.
Article in Microbial biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- Live and heat-treatediScience · 2026Article
- Influence of the pili ofApplied and environmental microbiology · 2025Article
- New Direction in Antimicrobial Delivery System: Preparation and Applications of Hydrogel Microspheres.Pharmaceutics · 2025Review
- Heat-KilledFoods (Basel, Switzerland) · 2023Article
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- Anti-salmonella properties of kefir yeast isolatesSaudi journal of biological sciences · 2022Article
- The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics.Nature reviews. Gastroenterology & hepatology · 2021Review
- Rehydration before Application Improves Functional Properties of LyophilizedMicroorganisms · 2021Article
- Lactic acid bacteria as probiotics for the nose?Microbial biotechnology · 2021Review
- The Possible Link Between Manufacturing and Probiotic Efficacy; a Molecular Point of View onFrontiers in microbiology · 2021Review
- A Fast, Efficient and Easy to Implement Method to Purify Bacterial Pili FromFrontiers in microbiology · 2020Article
- Impact of spray-drying on the pili of Lactobacillus rhamnosus GG.Microbial biotechnology · 2019Article
- Probiotics and prebiotics in clinical tests: an update.F1000Research · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preservation of the viability of microorganisms in probiotic formulations is the most important parameter ensuring the adequate concentration of live microorganisms at the time of administration. The formulation and processing techniques used to produce these probiotic formulations can influence the preservation of the microbial viability. However, it is also required that the bacteria maintain their key probiotic capacities during processing, formulation and shelf life. In this study, we investigated the impact of spray-drying on different cell wall properties of the model probiotic strain Lactobacillus rhamnosus GG, including its adherence to intestinal epithelial cells. The dltD gene knock-out mutant, L. rhamnosus GG CMPG5540, displaying modified cell wall lipoteichoic acids, showed significantly increased colony-forming units after spray-drying and subsequent storage under standard conditions compared to wild-type L. rhamnosus GG. In contrast, disruption of the biosynthesis of exopolysaccharides or pili expression did not impact survival. However, spray-drying did significantly affect the adherence capacity of L. rhamnosus GG. Scanning electron microscopy confirmed that the pili, key surface factors for adherence to intestinal cells and mucus, were sheared off during the spray-drying process. These data thus highlight that both the functionality and viability of probiotics should be assessed during the spray-drying process and subsequent storage.
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Registered trials
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