ArticleScience advances2019
AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Standardization of cross-site biophysical studies of bovine insulin amyloids is challenged by structural polymorphism.European biophysics journal : EBJ · 2026Article
- Zooming into Disease at the Nanoscale: High-Speed Atomic Force Microscopy in Biomedical Discovery.ACS nano · 2026Review
- Terahertz Imaging Detects Oral Cariogenic Microbial Domains Characteristics.Journal of dental research · 2024Article
- Time-resolved fluorescence of tryptophan characterizes membrane perturbation by cyclic lipopeptides.Biophysical journal · 2024Article
- Insights on the Mechanical Properties of SARS-CoV-2 Particles and the Effects of the Photosensitizer Hypericin.International journal of molecular sciences · 2024Article
- Review
- Expanding the microbiologist toolboxMicrobiology spectrum · 2024Article
- Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope.International journal of molecular sciences · 2023Article
- α-Synuclein fibril and synaptic vesicle interactions lead to vesicle destruction and increased lipid-associated fibril uptake into iPSC-derived neurons.Communications biology · 2023Article
- Label-free detection and identification of single bacteria via terahertz near-field imaging.Frontiers in microbiology · 2023Article
- From fixed-dried to wet-fixed to live - comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations.Nanophotonics (Berlin, Germany) · 2022Article
- Label-Free Characterization of Amyloids and Alpha-Synuclein Polymorphs by Exploiting Their Intrinsic Fluorescence Property.Analytical chemistry · 2022Article
- Super-resolution microscopy: a closer look at synaptic dysfunction in Alzheimer disease.Nature reviews. Neuroscience · 2021Review
- Correlative nanoscopy: A multimodal approach to molecular resolution.Microscopy research and technique · 2021Review
- Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy.Nature communications · 2021Article
- Challenges facing quantitative large-scale optical super-resolution, and some simple solutions.iScience · 2021Review
- Unveiling a Hidden Event in Fluorescence Correlative Microscopy by AFM Nanomechanical Analysis.Frontiers in molecular biosciences · 2021Article
- AFM-Based Correlative Microscopy Illuminates Human Pathogens.Frontiers in cellular and infection microbiology · 2021Review
- Article
- SSNOMBACTER: A collection of scattering-type scanning near-field optical microscopy and atomic force microscopy images of bacterial cells.GigaScience · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is known that the presence of fluorophores can influence the dynamics of molecular processes. Despite this, an affordable technique to control the fluorophore distribution within the sample, as well as the rise of unpredictable anomalous processes induced by the fluorophore itself, is missing. We coupled a stimulated emission depletion (STED) microscope with an atomic force microscope to investigate the formation of amyloid aggregates. In particular, we studied the in vitro aggregation of insulin and two alloforms of β amyloid peptides. We followed standard methods to induce the aggregation and to label the molecules at different dye-to-protein ratios. Only a fraction of the fibrillar aggregates was displayed in STED images, indicating that the labeled molecules did not participate indistinctly to the aggregation process. This finding demonstrates that labeled molecules follow only selected pathways of aggregation, among the multiple that are present in the aggregation reaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.