ArticleBiotechnology for biofuels2019
A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using
Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- In Situ Imaging for Monitoring Biomass Content and Pellet Size in Submerged Filamentous Stirred-Tank Cultivation.Biotechnology journal · 2026Article
- Understanding pellet population heterogeneity of Aspergillus niger in stirred tank and rocking motion bioreactors.Applied microbiology and biotechnology · 2026Article
- Co-cultivation dynamics of the filamentous microorganisms Aspergillus niger and Streptomyces coelicolor in shake flasks.Microbial cell factories · 2026Article
- Thermal and oxidative stress responses of Paecilomyces species recovered from beverage processing environments.World journal of microbiology & biotechnology · 2026Article
- Between FAMEs-Qualitative and Biomass-Quantitative: A review study on oleaginous fungi.Biotechnology notes (Amsterdam, Netherlands) · 2026Review
- Adjusting Aspergillus niger pellet diameter, population heterogeneity, and core architecture during shake flask cultivation.Biotechnology for biofuels and bioproducts · 2025Article
- Cultivation of filamentous fungi in airlift bioreactors: advantages and disadvantages.Applied microbiology and biotechnology · 2025Review
- Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization.BMC biotechnology · 2024Article
- Morphological Engineering of Filamentous Fungi: Research Progress and Perspectives.Journal of microbiology and biotechnology · 2024Review
- Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene.Applied microbiology and biotechnology · 2024Article
- Breaking down barriers: comprehensive functional analysis of the Aspergillus niger chitin synthase repertoire.Fungal biology and biotechnology · 2024Article
- Innovative Approaches to Fungal Food Production: Mycelial Pellet Morphology Insights.Foods (Basel, Switzerland) · 2023Article
- Regression modelling of conditional morphogene expression links and quantifies the impact of growth rate, fitness and macromorphology with protein secretion in Aspergillus niger.Biotechnology for biofuels and bioproducts · 2023Article
- Recent Advances in Chitin Biosynthesis Associated with the Morphology and Secondary Metabolite Synthesis of Filamentous Fungi in Submerged Fermentation.Journal of fungi (Basel, Switzerland) · 2023Review
- Computation-aided studies related to the induction of specialized metabolite biosynthesis in microbial co-cultures: An introductory overview.Computational and structural biotechnology journal · 2023Review
- Quantitative phenotypic screens of Aspergillus niger mutants in solid and liquid culture.STAR protocols · 2022Article
- Protocol for gene characterization in Aspergillus niger using 5S rRNA-CRISPR-Cas9-mediated Tet-on inducible promoter exchange.STAR protocols · 2022Article
- Cephalosporin C biosynthesis and fermentation in Acremonium chrysogenum.Applied microbiology and biotechnology · 2022Review
- Comprehensively dissecting the hub regulation of PkaC on high-productivity and pellet macromorphology in citric acid producing Aspergillus niger.Microbial biotechnology · 2022Article
- CRISPR/Cas9-Based Genome Editing and Its Application inJournal of fungi (Basel, Switzerland) · 2022Review
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including dispersed mycelia, clumped aggregates, or pellets, which have critical implications for rheological aspects during fermentation, gas/nutrient transfer, and, thus, product titres. An important component of strain engineering efforts is the ability to quantitatively assess fungal growth phenotypes, which will drive novel leads for morphologically optimized production strains.
resultsIn this study, we developed an automated image analysis pipeline to quantify the morphology of pelleted and dispersed growth (MPD) which rapidly and reproducibly measures dispersed and pelleted macromorphologies from any submerged fungal culture. It (i) enables capture and analysis of several hundred images per user/day, (ii) is designed to quantitatively assess heterogeneous cultures consisting of dispersed and pelleted forms, (iii) gives a quantitative measurement of culture heterogeneity, (iv) automatically generates key Euclidian parameters for individual fungal structures including particle diameter, aspect ratio, area, and solidity, which are also assembled into a previously described dimensionless morphology number MN, (v) has an in-built quality control check which enables end-users to easily confirm the accuracy of the automated calls, and (vi) is easily adaptable to user-specified magnifications and macromorphological definitions. To concomitantly provide proof of principle for the utility of this image analysis pipeline, and provide new leads for morphologically optimized fungal strains, we generated a morphological mutant in the cell factory
conclusionThe MPD image analysis pipeline is a simple, rapid, and flexible approach to quantify diverse fungal morphologies. As an exemplar, we have demonstrated that the putative endosomal transport gene
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.