Evidence map›Paper›PMID 31223178›Full record

ArticleNuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment2018

Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy.

Antonino Ingargiola, Maya Segal, Angelo Gulinatti, Ivan Rech, Ivan Labanca, Piera Maccagnani, Massimo Ghioni, Shimon Weiss, Xavier Michalet

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Antonino IngargiolaDepartment of Chemistry and Biochemistry, University of California Los Angeles, USA.
Maya SegalDepartment of Chemistry and Biochemistry, University of California Los Angeles, USA.
Angelo GulinattiDip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
Ivan RechDip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
Ivan LabancaDip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
Piera MaccagnaniIstituto per la Microelettronica e Microsistemi, IMM-CNR, Bologna, Italy.
Massimo GhioniDip. di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Milan, Italy.
Shimon WeissDepartment of Chemistry and Biochemistry, University of California Los Angeles, USA.
Xavier MichaletDepartment of Chemistry and Biochemistry, University of California Los Angeles, USA.
Politecnico di Milano · ITUniversity of California, Los Angeles · USInstitute for Microelectronics and Microsystems · IT

Funding

Single-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0M
NIGMS NIH HHS R01 GM095904
6 · The paper itself

Abstract

Single-molecule fluorescence spectroscopy (SMFS), based on the detection of individual molecules freely diffusing through the excitation spot of a confocal microscope, has allowed unprecedented insights into biological processes at the molecular level, but suffers from limited throughput. We have recently introduced a multispot version of SMFS, which allows achieving high-throughput SMFS by virtue of parallelization, and relies on custom silicon single-photon avalanche diode (SPAD) detector arrays. Here, we examine the premise of this parallelization approach, which is that data acquired from different spots is uncorrelated. In particular, we measure the optical crosstalk characteristics of the two 48-pixel SPAD arrays used in our recent SMFS studies, and demonstrate that it is negligible (crosstalk probability ≤ 1.1 10

Indexed as

FluorescenceOptical crosstalkSingle-moleculeSPAD arraySpectroscopy

Identifiers

PMID31223178
PMCPMC6586236
OpenAlexW2953198216

What OpenQuestion holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.