ArticleBMC complementary and alternative medicine2019
ERK/Nrf2 pathway activation by caffeic acid in HepG2 cells alleviates its hepatocellular damage caused by t-butylhydroperoxide-induced oxidative stress.
Article in BMC complementary and alternative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 73 citations in OpenAlex.
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- Protective effects of carotenoids against blue light induced-cellular damage in human retinal pigment epithelium.Food science and biotechnology · 2025Article
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- Phenolic acids in fermented foods: microbial biotransformation, antioxidant mechanisms, and functional health implications.Frontiers in molecular biosciences · 2025Review
- Carboxylic Acids as Activators of NRF2: Antioxidant and Anti-inflammatory Effects.Medicinal chemistry (Shariqah (United Arab Emirates)) · 2025Review
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Adjuvant Properties of Caffeic Acid in Cancer Treatment.International journal of molecular sciences · 2024Review
- Analysis of Lemon Verbena Polyphenol Metabolome and Its Correlation with Oxidative Stress under Glucotoxic Conditions in Adipocyte.Journal of agricultural and food chemistry · 2024Article
- Review
- Combined effects of green tea supplementation and eccentric exercise on nuclear factor erythroid 2-related factor 2 activity.European journal of applied physiology · 2024Article
- Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage.Biochemistry and biophysics reports · 2023Article
- Unlocking the Potential: Novel NSAIDs Hybrids Unleash Chemopreventive Power toward Liver Cancer Cells through Nrf2, NF-κB, and MAPK Signaling Pathways.Molecules (Basel, Switzerland) · 2023Article
- Phytochemical Screening and Antioxidant Potential of Selected Extracts fromPlants (Basel, Switzerland) · 2023Article
- Role of Plant-Derived Compounds in the Molecular Pathways Related to Inflammation.International journal of molecular sciences · 2023Review
- Genistein suppresses microglial activation and inhibits apoptosis in different brain regions of hypoxia-exposed mice model of amnesia.Metabolic brain disease · 2022Article
- Potential ofMolecules (Basel, Switzerland) · 2022Review
- Comparison of the Antihypertensive Activity of Phenolic Acids.Molecules (Basel, Switzerland) · 2022Article
- Antioxidative properties of phenolic compounds and their effect on oxidative stress induced by severe physical exercise.The journal of physiological sciences : JPS · 2022Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundSeveral studies have found that caffeic acid (CA), a well-known phytochemical, displays important antioxidant and anti-cancer activities. However, no evidence exists on the protective effect and its mechanisms that CA treatment alone has against oxidative stress induced by tert-butyl hydroperoxide (t-BHP) in HepG2 cells.
methodsHepatoprotective activities such as cell viability, mRNA expression, and report gene assay were measured using HepG2 cell. Three types of genes and proteins related with detoxification in liver were used for measuring the hepatoprotective effects. Statistical analysis was performed using one-way ANOVA test and differences among groups were evaluated by Tukey's studentized range tests.
resultsThe present study indicate that treatment with CA up-regulates heme oxygenase-1 (HO-1) and glutamate-cysteine ligase (GCL) mRNA and protein expressions in a CA-dose-dependent manner. In addition, translocation of nuclear factor-E2 p45-related factor (Nrf2) from the cytoplasm to the nucleus and phosphorylation of extracellular signal-regulated kinase, ERK and c-Jun N-terminal kinase, JNK which have been shown to be involved in mitogen-activated protein kinases, MAPKs are significantly enhanced by CA treatment. Furthermore, in cell nuclei, CA enhances the 5'-flanking regulatory region of human antioxidant response element (ARE) and activates the ARE binding site.
conclusionTherefore, CA proved to be a stimulant of the expression of detoxification enzymes such as HO-1, GCLC, and GCLM through the ERK/Nrf2 pathway, and it may be an effective chemoprotective agent for protecting liver damage against oxidative damage.
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