ArticleThe journals of gerontology. Series A, Biological sciences and medical sciences2020
The Effects of Graded Levels of Calorie Restriction: XIV. Global Metabolomics Screen Reveals Brown Adipose Tissue Changes in Amino Acids, Catecholamines, and Antioxidants After Short-Term Restriction in C57BL/6 Mice.
Article in The journals of gerontology. Series A, Biological sciences and medical sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Mild Short-Term Caloric Restriction Induces Coordinated Changes That Promote Lipid Deposition While Maintaining Thermogenesis Capacity in Interscapular Brown Adipose Tissue of Male Rats.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Less is more: calorie restriction as a therapeutic for mental health disorders.Frontiers in psychiatry · 2025Review
- Does diet influence aging? Evidence from animal studies.Journal of internal medicine · 2024Review
- Diet Restriction Impact on High-Fat-Diet-Induced Obesity by Regulating Mitochondrial Cardiolipin Biosynthesis and Remodeling.Molecules (Basel, Switzerland) · 2023Article
- Transcriptomics Dissection of Calorie Restriction and Exercise Training in Brown Adipose Tissue and Skeletal Muscle.Nutrients · 2023Article
- Thermogenic Adipose Redox Mechanisms: Potential Targets for Metabolic Disease Therapies.Antioxidants (Basel, Switzerland) · 2023Review
- The Second Annual Symposium of the Midwest Aging Consortium: The Future of Aging Research in the Midwestern United States.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Article
- Fasting drives the metabolic, molecular and geroprotective effects of a calorie-restricted diet in mice.Nature metabolism · 2021Article
- Strain-specific metabolic responses to long-term caloric restriction in female ILSXISS recombinant inbred mice.Molecular and cellular endocrinology · 2021Review
- The effects of graded calorie restriction XVII: Multitissue metabolomics reveals synthesis of carnitine and NAD, and tRNA charging as key pathways.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Energy Metabolism and Aging.The world journal of men's health · 2021Review
- Integrated metabolomics reveals altered lipid metabolism in adipose tissue in a model of extreme longevity.GeroScience · 2020Article
- Healthful aging mediated by inhibition of oxidative stress.Ageing research reviews · 2020Review
- From a "Metabolomics fashion" to a sound application of metabolomics in research on human nutrition.European journal of clinical nutrition · 2020Review
- Deletion of Nrf2 shortens lifespan in C57BL6/J male mice but does not alter the health and survival benefits of caloric restriction.Free radical biology & medicine · 2020Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Animals undergoing calorie restriction (CR) often lower their body temperature to conserve energy. Brown adipose tissue (BAT) is stimulated through norepinephrine when rapid heat production is needed, as it is highly metabolically active due to the uncoupling of the electron transport chain from ATP synthesis. To better understand how BAT metabolism changes with CR, we used metabolomics to identify 883 metabolites that were significantly differentially expressed in the BAT of C57BL/6 mice, fed graded CR (10%, 20%, 30%, and 40% CR relative to their individual baseline intake), compared with mice fed ad libitum (AL) for 12 hours a day. Pathway analysis revealed that graded CR had an impact on the TCA cycle and fatty acid degradation. In addition, an increase in nucleic acids and catecholamine pathways was seen with graded CR in the BAT metabolome. We saw increases in antioxidants with CR, suggesting a beneficial effect of mitochondrial uncoupling. Importantly, the instigator of BAT activation, norepinephrine, was increased with CR, whereas its precursors l-tyrosine and dopamine were decreased, indicating a shift of metabolites through the activation pathway. Several of these key changes were correlated with food anticipatory activity and body temperature, indicating BAT activation may be driven by responses to hunger.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.