ArticlePloS one2019
Assessment of reproducibility and biological variability of fasting and postprandial plasma metabolite concentrations using 1H NMR spectroscopy.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Harnessing metabolomics and proteomics in a clinical trial for pulmonary arterial hypertension: insights from post-hoc analysis of the REHAB-PH trial.EBioMedicine · 2026Trial
- Impact of comprehensive lifestyle interventions on plasma branched-chain amino acid concentrations: a randomized trial.The American journal of clinical nutrition · 2025Trial
- Metabolic Markers of MRI-Confirmed Lacunar Stroke: Observational and Mendelian Randomization Analyses.Neurology · 2026Observational
- A high-resolution circulating metabolic atlas of lung adenocarcinoma progression supports early and accurate diagnosis.Cell reports. Medicine · 2026Article
- Value of Multiomics Over Clinical Risk Factors in Hypertension Prediction.Hypertension (Dallas, Tex. : 1979) · 2026Article
- Metabolomics biomarkers for precision psychiatry.Frontiers in psychiatry · 2026Review
- Reproducibility and Sources of Variation of Urinary Biomarkers of Food Intake of Fruits, Vegetables, and Chocolate in European Children and Adolescents.The Journal of nutrition · 2026Article
- Novel type 2 diabetes prediction score based on traditional risk factors and circulating metabolites: model derivation and validation in two large cohort studies.EClinicalMedicine · 2025Article
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- Associations of childhood BMI, general and visceral fat mass with metabolite profiles at school-age.International journal of obesity (2005) · 2024Article
- Dietary biomarkers-an update on their validity and applicability in epidemiological studies.Nutrition reviews · 2024Review
- Circulating fatty acids and risk of hepatocellular carcinoma and chronic liver disease mortality in the UK Biobank.Nature communications · 2024Article
- Article
- Associations of circulating fatty acids with incident coronary heart disease: a prospective study of 89,242 individuals in UK Biobank.BMC cardiovascular disorders · 2023Article
- NMR-Metabolomics Reveals a Metabolic Shift after Surgical Resection of Non-Small Cell Lung Cancer.Cancers · 2023Article
- Metabolic profiling of MRI-measured liver fat in the UK Biobank.Obesity (Silver Spring, Md.) · 2023Article
- Single and Joined Behaviour of Circulating Biomarkers and Metabolic Parameters in High-Fit and Low-Fit Healthy Females.International journal of molecular sciences · 2023Article
- Predictive value of circulating NMR metabolic biomarkers for type 2 diabetes risk in the UK Biobank study.BMC medicine · 2022Article
- Maternal Body Mass Index, Early-Pregnancy Metabolite Profile, and Birthweight.The Journal of clinical endocrinology and metabolism · 2022Observational
- Spousal associations of serum metabolomic profiles by nuclear magnetic resonance spectroscopy.Scientific reports · 2021Article
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9 authors.
Funding
Abstract
introductionIt is crucial to understand the factors that introduce variability before applying metabolomics to clinical and biomarker research.
objectivesWe quantified technical and biological variability of both fasting and postprandial metabolite concentrations measured using 1H NMR spectroscopy in plasma samples.
methodsIn the Netherlands Epidemiology of Obesity study (n = 6,671), 148 metabolite concentrations (101 metabolites belonging to lipoprotein subclasses) were measured under fasting and postprandial states (150 minutes after a mixed liquid meal). Technical variability was evaluated among 265 fasting and 851 postprandial samples, with the identical blood plasma sample being measured twice by the same laboratory protocol. Biological reproducibility was assessed by measuring 165 individuals twice across time for evaluation of short- (<6 months) and long-term (>3 years) biological variability. Intra-class coefficients (ICCs) were used to assess variability. The ICCs of the fasting metabolites were compared with the postprandial metabolites using two-sided paired Wilcoxon test separately for short- and long-term measurements.
resultsBoth fasting and postprandial metabolite concentrations showed high technical reproducibility using 1H NMR spectroscopy (median ICC = 0.99). Postprandial metabolite concentrations revealed slightly higher ICC scores than fasting ones in short-term repeat measures (median ICC in postprandial and fasting metabolite concentrations 0.72 versus 0.67, Wilcoxon p-value = 8.0×10-14). Variability did not increase further in a long-term repeat measure, with median ICC in postprandial of 0.64 and in fasting metabolite concentrations 0.66.
conclusionTechnical reproducibility is excellent. Biological reproducibility of postprandial metabolite concentrations showed a less or equal variability than fasting metabolite concentrations over time.
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