ArticlePloS one2019
Increased cerebral vascularization and decreased water exchange across the blood-brain barrier in aquaporin-4 knockout mice.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Increased neurotoxic gliosis and blood-brain barrier dysregulation in canine cognitive dysfunction syndrome (CCD).Frontiers in aging · 2026Article
- Aquaporins in Acute Brain Injury: Insights from Clinical and Experimental Studies.Biomedicines · 2025Review
- Device-assisted strategies for drug delivery across the blood-brain barrier to treat glioblastoma.Communications materials · 2025Review
- MRI detects blood-brain barrier alterations in a rat model of Alzheimer's disease and lung infection.Npj imaging · 2025Article
- Loss of aquaporin-4 impairs cerebrospinal fluid solute clearance through cerebrospinal fluid drainage pathways.Scientific reports · 2024Article
- Article
- Age-Related Decline in Blood-Brain Barrier Function is More Pronounced in Males than Females in Parietal and Temporal Regions.bioRxiv : the preprint server for biology · 2024Article
- MRI free water mediates the association between water exchange rate across the blood brain barrier and executive function among older adults.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Loss of aquaporin-4 results in glymphatic system dysfunction via brain-wide interstitial fluid stagnation.eLife · 2023Article
- Ion Channels in Gliomas-From Molecular Basis to Treatment.International journal of molecular sciences · 2023Review
- Aquaporins in Nervous System.Advances in experimental medicine and biology · 2023Article
- Whole-brain perfusion mapping in mice by dynamic BOLD MRI with transient hypoxia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Cholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling.BMC neuroscience · 2022Article
- Fluid transport in the brain.Physiological reviews · 2022Review
- Distribution of intraperitoneally administered deuterium-labeled water in aquaporin-4-knockout mouse brain after middle cerebral artery occlusion.Frontiers in neuroscience · 2022Article
- Exploring cell membrane water exchange in aquaporin-4-deficient ischemic mouse brain using diffusion-weighted MRI.European radiology experimental · 2021Article
- Revisiting Astrocytic Roles in Methylmercury Intoxication.Molecular neurobiology · 2021Review
- Dynamic oxygen-17 MRI with adaptive temporal resolution using golden-means-based 3D radial sampling.Magnetic resonance in medicine · 2021Article
- Activation and Role of Astrocytes in Ischemic Stroke.Frontiers in cellular neuroscience · 2021Review
- Remodelling and Treatment of the Blood-Brain Barrier in Glioma.Cancer management and research · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Aquaporin-4 (AQP4) plays an important role in regulating water exchange across the blood-brain barrier (BBB) and brain-cerebrospinal fluid interface. Studies on AQP-4 knockout mice (AQP4-KO) have reported considerable protection from brain edema induced by acute water intoxication and ischemic stroke, identifying AQP4 as a potential target for therapeutic interventions. However, the long-term effects of chronic AQP4 suppression are yet to be elucidated. In the current study, we evaluated the physiological and structural changes in adult AQP4-KO mice using magnetic resonance imaging (MRI) and immunohistochemical analysis. Water exchange across BBB was assessed by tracking an intravenous bolus injection of oxygen-17 (17O) water (H217O) using 17O-MRI. Cerebral blood flow (CBF) was quantified using arterial spin-labeling (ASL) MRI. Capillary density was determined by immunohistochemical staining for glucose transporter-1 (GLUT1). Compared to wildtype control mice, AQP4-KO mice showed a significant reduction in peak and steady-state H217O uptake despite unaltered CBF. Interestingly, a 22% increase in cortical capillary density was observed in AQP4-KO mice. These results suggest that increased cerebral vascularization may be an adaptive response to chronic reduction in water exchange across BBB in AQP4-KO mice.
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Registered trials
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