ArticleThe Journal of clinical investigation2019
Bystander responses impact accurate detection of murine and human antigen-specific CD8 T cells.
Article in The Journal of clinical investigation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 30 citations in OpenAlex.
- Pre-existing Immunity to Japanese Encephalitis Virus Alters CD4 T Cell Responses to Zika Virus Inactivated Vaccine.Frontiers in immunology · 2021Trial
- Human lungs maintain tissue-resident memory T cells against a broad spectrum of pathogens.Nature immunology · 2026Article
- The challenge and promise of studying human antigen-specific T cells.Nature reviews. Immunology · 2026Review
- Review
- Antigen-specific TScience advances · 2026Article
- The ability of memory CD8 T cell subsets to numerically and functionally recover following whole body irradiation is influenced by their history of cognate antigen exposures.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Cytokine and metabolite networks shape T cell residency and functionality at the term human maternal-fetal interface.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Anti-HER2/neu TCR-T Cells in Action: linking transcriptional signatures, secretomics, andFrontiers in immunology · 2025Article
- Steady-state, therapeutic, and helminth-induced IL-4 compromise protective CD8 T cell bystander activation.bioRxiv : the preprint server for biology · 2024Article
- Signals that control MAIT cell function in healthy and inflamed human tissues.Immunological reviews · 2024Review
- Adverse outcomes and an immunosuppressed endotype in septic patients with reduced IFN-γ ELISpot.JCI insight · 2024Article
- IL-15-induced CD38Clinical & translational immunology · 2024Article
- Sepsis leads to lasting changes in phenotype and function of naïve CD8 T cells.PLoS pathogens · 2023Article
- Antigen-specificity measurements are the key to understanding T cell responses.Frontiers in immunology · 2023Review
- Sepsis-induced changes in differentiation, maintenance, and function of memory CD8 T cell subsets.Frontiers in immunology · 2023Review
- Convergent clonal selection of donor- and recipient-derived CMV-specific T cells in hematopoietic stem cell transplant patients.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Adaptive Immune Receptor Repertoire (AIRR) Community Guide to Repertoire Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Inflammatory signals are sufficient to elicit TOX expression in mouse and human CD8+ T cells.JCI insight · 2021Article
- Prolonged Reactive Oxygen Species Production following Septic Insult.ImmunoHorizons · 2021Observational
- Cutting Edge: Antitumor Immunity by Pathogen-Specific CD8 T Cells in the Absence of Cognate Antigen Recognition.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Induction of memory CD8 T cells is important for controlling infections such as malaria HIV/AIDS, and for cancer immunotherapy. Accurate assessment of antigen (Ag)-specific CD8 T-cells is critical for vaccine optimization and defining correlates of protection. However, conditions for determining Ag-specific CD8 T-cell responses ex-vivo using ICS may be variable, especially in humans with complex antigens. Here, we used an attenuated whole parasite malaria vaccine model in humans and various experimental infections in mice to show that the duration of antigenic stimulation and timing of brefeldin A (BFA) addition influences the magnitude of Ag-specific and bystander T cell responses. Indeed, following immunization with an attenuated whole sporozoite malaria vaccine in humans, significantly higher numbers of IFN-γ producing memory CD8 T-cells comprised of antigen specific and bystander responses were detected by increasing the duration of Ag-stimulation prior to addition of BFA. Mechanistic analyses of virus-specific CD8 T-cells in mice revealed that the increase in IFNg producing CD8 T-cells was due to bystander activation of Ag-experienced memory CD8 T-cells, and correlated with the proportion of Ag-experienced CD8 T-cells in the stimulated populations. Incubation with anti-cytokine antibodies (ex. IL-12) improved accuracy in detecting bona-fide memory CD8 T-cell responses suggesting this as the mechanism for the bystander activation. These data have important implications for accurate assessment of immune responses generated by vaccines intended to elicit protective memory CD8 T-cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.