ArticleJournal of industrial microbiology & biotechnology2019
Combining metabolic flux analysis and adaptive evolution to enhance lipase production in Bacillus subtilis.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 14 citations in OpenAlex.
- Genetically engineered lipases: advances in expression and upscaling for industrial applications.Archives of microbiology · 2026Review
- Engineering Bacillus subtilis J46 for efficient utilization of galactose through adaptive laboratory evolution.AMB Express · 2024Article
- Recent progress in adaptive laboratory evolution of industrial microorganisms.Journal of industrial microbiology & biotechnology · 2023Article
- Enhancing menaquinone-7 biosynthesis by adaptive evolution of Bacillus natto through chemical modulator.Bioresources and bioprocessing · 2022Article
- Harnessing microbial metabolomics for industrial applications.World journal of microbiology & biotechnology · 2019Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Metabolic fluxes during lipase production by Bacillus subtilis CICC 20034 in synthetic medium were studied using metabolic flux analysis (MFA). The MFA showed that lipase production was dependent on, and coupled to the tributyrin uptake rate, formation of biomass, lactate, ATP, as well as amino acids from the aspartate and glutamate family. Using tributyrin as the sole carbon source, an adaptive evolution strategy was applied to increase the tributyrin uptake rate. B. subtilis SPZ1 was obtained from CICC 20034 by adaptive evolution over 1000 generations of growth-based selection. The tributyrin consumption rate of strain SPZ1 reached 0.89 g/(L·h) which was 1.9-fold higher than that of the original strain. The MFA indicated that the 212% increase of tributyrin uptake flux contributed to the 556% increase of lipase flux. Consequently, the lipase activity (0.65 U/mL) of strain SPZ1 was 1.9-fold higher than that of the original strain. This was the highest lipase activity obtained by fermentation in synthetic medium reported for Bacillus strains. In complex culture medium, lipase activity of SPZ1 reached 3.3 U/mL.
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