ArticleApplied microbiology and biotechnology2019
The filamentous fungus Penicillium chrysogenum analysed via flow cytometry-a fast and statistically sound insight into morphology and viability.
Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Reliable online measurement of population dynamics for filamentous co-cultures.Microbial biotechnology · 2022Article
- Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia.Microbial cell factories · 2022Article
- Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi.Applied microbiology and biotechnology · 2022Article
- Rapid and concise quantification of mycelial growth by microscopic image intensity model and application to mass cultivation of fungi.Scientific reports · 2021Article
- Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems-A critical review.Engineering in life sciences · 2021Review
- Advances in monitoring and control of refolding kinetics combining PAT and modeling.Applied microbiology and biotechnology · 2021Review
- A robust flow cytometry-based biomass monitoring tool enables rapid at-line characterization of S. cerevisiae physiology during continuous bioprocessing of spent sulfite liquor.Analytical and bioanalytical chemistry · 2020Article
- Optimal process design space to ensure maximum viability and productivity in Penicillium chrysogenum pellets during fed-batch cultivations through morphological and physiological control.Microbial cell factories · 2020Article
- Article
- Development of Novel Bioreactor Control Systems Based on Smart Sensors and Actuators.Frontiers in bioengineering and biotechnology · 2020Review
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Authors and funding
2 authors.
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Abstract
Filamentous fungi serve as production host for a number of highly relevant biotechnological products, like penicillin. In submerged culture, morphology can be exceptionally diverse and is influenced by several process parameters, like aeration, agitation, medium composition or growth rate. Fungal growth leads to several morphological classes encompassing homogeneously dispersed hyphae and various forms of hyphal agglomerates and/or clump structures. Eventually, the so-called pellet structure can be formed, which represents a hyphal agglomerate with a dense core. Pellet structures can hinder oxygen and substrate transport, resulting in different states of viability, which in turn affects productivity and process control. Over the years, several publications have dealt with methods to either gain morphological insight into pellet structure or determine biomass viability. Within this contribution, we present a way to combine both in a flow cytometry-based method employing fluorescent staining. Thereby, we can assess filamentous biomass in a statistically sound way according to (i) morphology and (ii) viability of each detected morphological form. We are confident that this method can shed light on the complex relationship between fungal morphology, viability and productivity-in both process development and routine manufacturing processes.
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