ArticleScientific reports2019
Development of CS-TPP-dsRNA nanoparticles to enhance RNAi efficiency in the yellow fever mosquito, Aedes aegypti.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 144 citations in OpenAlex.
- Synthetic polymer nanocarrier enhances dsRNA stability but not RNAi efficacy in Brassicogethes aeneus.Pest management science · 2026Article
- Comparative evaluation of chitosan-based and star polycation nanocarriers for enhanced RNAi efficacy targeting CmFibL in Cnaphalocrocis medinalis.Pest management science · 2026Article
- Biogenic nanomaterials: A green strategy for sustainable vector-borne disease management.Pest management science · 2026Review
- EngineeredInsects · 2026Article
- COF-PEI-PEG nanocarriers enhance dsRNA delivery to improve RNAi-based insect pest control.Pest management science · 2026Article
- Chitosan-Tripolyphosphate Nanoparticles Enhance Foliar dsRNA Delivery and Suppress Turnip Crinkle Virus in Arabidopsis.The plant pathology journal · 2026Article
- Chitosan-protected dsRNA increases efficacy against Helicoverpa armigera without compromising biosafety for pollinators.Pest management science · 2026Article
- Article
- Bibliometric trends and emerging frontiers in RNA interference research for mosquito control (2010-2025).Frontiers in insect science · 2026Review
- Overview of polymeric polyplexes for dsRNA delivery in insects: complexation, stability, and design considerations.Frontiers in insect science · 2026Review
- Evaluation of dsRNA produced inFrontiers in insect science · 2026Article
- RNA interference mediated mortality in Aedes albopictus: a challenging journey toward species-specific vector control.Parasites & vectors · 2025Article
- Improving cisplatin chemotherapy in vivo by niosomal propolis and chrysin as nanoadjuvants.Medical oncology (Northwood, London, England) · 2025Article
- RNA Interference-Mediated Silencing ofInternational journal of molecular sciences · 2025Article
- ExploitingPathogens (Basel, Switzerland) · 2025Review
- Targeting Respiratory Viruses: The Efficacy of Intranasal mRNA Vaccination in Generating Protective Mucosal and Systemic Immunity Against Influenza A (H1N1).Influenza and other respiratory viruses · 2025Article
- Preparation ofInsects · 2024Article
- Nanocarrier-Based Eco-Friendly RNA Pesticides for Sustainable Management of Plant Pathogens and Pests.Nanomaterials (Basel, Switzerland) · 2024Review
- The role of polymers in enabling RNAi-based technology for sustainable pest management.Nature communications · 2024Review
- Research Progress on miRNAs and Artificial miRNAs in Insect and Disease Resistance and Breeding in Plants.Genes · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. Vector control is one of the most important approaches used in reducing the incidence of these diseases. However, increasing mosquito resistance to chemical insecticides presents challenges to this approach. Therefore, new strategies are necessary to develop the next generation vector control methods. Because of the target specificity of dsRNA, RNAi-based control measures are an attractive alternative to current insecticides used to control disease vectors. In this study, Chitosan (CS) was cross-linked to sodium tripolyphosphate (TPP) to produce nano-sized polyelectrolyte complexes with dsRNA. CS-TPP-dsRNA nanoparticles were prepared by ionic gelation method. The encapsulation efficiency, protection of dsRNA from nucleases, cellular uptake, in vivo biodistribution, larval mortality and gene knockdown efficiency of CS-TPP-dsRNA nanoparticles were determined. The results showed that at a 5:1 weight ratio of CS-TPP to dsRNA, nanoparticles of less than 200 nm mean diameter and a positive surface charge were formed. Confocal microscopy revealed the distribution of the fed CS-TPP-dsRNA nanoparticles in midgut, fat body and epidermis of yellow fever mosquito, Aedes aegypti larvae. Bioassays showed significant mortality of larvae fed on CS-TPP-dsRNA nanoparticles. These assays also showed knockdown of a target gene in CS-TPP-dsRNA nanoparticle fed larvae. These data suggest that CS-TPP nanoparticles may be used for delivery of dsRNA to mosquito larvae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.