ArticleScientific reports2019
Sniffer cells for the detection of neural Angiotensin II in vitro.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 10 citations in OpenAlex.
- Spontaneous and evoked angiotensin II sniffer cell activity in the lamina terminalis in vitro.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Sodium Intake and Disease: Another Relationship to Consider.Nutrients · 2023Review
- Role of angiotensin II in chronic intermittent hypoxia-induced hypertension and cognitive decline.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Neuropeptide release in the brain has traditionally been difficult to observe. Existing methods lack temporal and spatial resolution that is consistent with the function and size of neurons. We use cultured "sniffer cells" to improve the temporal and spatial resolution of observing neuropeptide release. Sniffer cells were created by stably transfecting Chinese Hamster Ovary (CHO) cells with plasmids encoding the rat angiotensin type 1a receptor and a genetically encoded Ca2+ sensor. Isolated, cultured sniffer cells showed dose-dependent increases in fluorescence in response to exogenously applied angiotensin II and III, but not other common neurotransmitters. Sniffer cells placed on the median preoptic nucleus (a presumptive site of angiotensin release) displayed spontaneous activity and evoked responses to either electrical or optogenetic stimulation of the subfornical organ. Stable sniffer cell lines could be a viable method for detecting neuropeptide release in vitro, while still being able to distinguish differences in neuropeptide concentration.
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Registered trials
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