Evidence map›Paper›PMID 31217279›Full record

ArticleThe Journal of biological chemistry2019

Hepatic steroid sulfatase critically determines estrogenic activities of conjugated equine estrogens in human cells

Ye Feng, Yang Xie, Meishu Xu, Linhao Li, Kyle W Selcer, Patrick J Oberly, Samuel M Poloyac, Hongbing Wang, Chengjiang Li, Fengqin Dong and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Ye FengCenter for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261; Department of Endocrinology and Metabolic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Yang XieCenter for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
Meishu XuCenter for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
Linhao LiDepartment of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201.
Kyle W SelcerDepartment of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282.
Patrick J OberlyDepartment of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
Samuel M PoloyacDepartment of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261.
Hongbing WangDepartment of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, Maryland 21201.
Chengjiang LiDepartment of Endocrinology and Metabolic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Fengqin DongDepartment of Endocrinology and Metabolic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Chaohui YuDepartment of Gastroenterology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. Electronic address: zyyyych@zju.edu.cn.
Wen XieCenter for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15261; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261. Electronic address: wex6@pitt.edu.
University of Pittsburgh · USFirst Affiliated Hospital Zhejiang University · CNUniversity of Maryland, Baltimore · USDuquesne University · USZhejiang University · CN

Funding

The AHR-FGF221 Axis in Hepatic Steatosis and Metobolic SyndromeR01DK083952 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI XIE, WEN · 2010 to 2019
$3.0M
A Novel Regulation of the Phase II Enzyme Estrogen SulfotransferaseR01ES023438 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI XIE, WEN · 2014 to 2018
$1.7M
Function and Regulation of SLC13A5 in the LiverR01GM121550 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI WANG, HONGBING · 2018 to 2021
$1.3M
NIDDK NIH HHS R01 DK083952NIEHS NIH HHS R01 ES023438NIGMS NIH HHS R01 GM121550
6 · The paper itself

Abstract

Conjugated equine estrogens (CEEs), whose brand name is Premarin, are widely used as a hormone-replacement therapy (HRT) drug to manage postmenopausal symptoms in women. Extracted from pregnant mare urine, CEEs are composed of nearly a dozen estrogens existing in an inactive sulfated form. To determine whether the hepatic steroid sulfatase (STS) is a key contributor to the efficacy of CEEs in HRT, we performed estrogen-responsive element (ERE) reporter gene assay, real-time PCR, and UPLC-MS/MS to assess the STS-dependent and inflammation-responsive estrogenic activity of CEEs in HepG2 cells and human primary hepatocytes. Using liver-specific STS-expressing transgenic mice, we also evaluated the effect of STS on the estrogenic activity of CEEs

Indexed as

AnimalsCells, CulturedChromatography, High Pressure LiquidEstrogen Receptor alphaEstrogens, Conjugated (USP)FemaleHepatocytesHorsesHumansInflammationLiverMiceMice, Inbred C57BLMice, TransgenicRNA InterferenceRNA, Small InterferingEstrogen Receptor alphaEstrogens, Conjugated (USP)RNA, Small InterferingSteryl-Sulfataseconjugated equine estrogensestrogengene regulationhormone replacement therapy (HRT)inflammationmenopausemetabolismsteroid hormonesteroid sulfatase (STS)sulfotransferase

Identifiers

PMID31217279
PMCPMC6690699
OpenAlexW2950364698

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.