Evidence map›Paper›PMID 31216394›Full record

ArticleCurrent protocols in neuroscience2019

Magnetic Cell Sorting for In Vivo and In Vitro Astrocyte, Neuron, and Microglia Analysis.

Leanne M Holt, S Tristan Stoyanof, Michelle L Olsen

Open access · greenAbstract read
In one paragraph

Article in Current protocols in neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 68 citations in OpenAlex.

  1. Article
  2. Astrocyte TrkB.T1 Deficiency Disrupts Glutamatergic Synaptogenesis and Astrocyte-Synapse Interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026
    Article
  3. Review
  4. Programmable nanobody circuits for cell selection.bioRxiv : the preprint server for biology · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Leanne M HoltDepartment of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama.
S Tristan StoyanofSchool of Neuroscience, Virginia Polytechnic and State University, Blacksburg, Virginia.
Michelle L OlsenSchool of Neuroscience, Virginia Polytechnic and State University, Blacksburg, Virginia.
Virginia Tech · US

Funding

Epigenetic Regulation of Kir4.1 and GLT1 in PathophysiologyR01NS075062 · NINDS · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI OLSEN, MICHELLE L · 2013 to 2017
$1.6M
Astrocytes are a primary target of neuronal-derived BDNF: a novel mechanism for dysfunction in Rett SyndromeF31NS100259 · NINDS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI HOLT, LEANNE · 2017 to 2019
$134k
NINDS NIH HHS F31 NS100259NINDS NIH HHS R01 NS075062
6 · The paper itself

Abstract

Interest in evaluating individual cellular populations in the central nervous system has prompted the development of several techniques enabling the enrichment of single-cell populations. Herein we detail a relatively inexpensive method to specifically isolate neurons, astrocytes, and microglia from a mixed homogenate utilizing magnetic beads conjugated to cell-type specific antibodies. We have used this technique to isolate astrocytes across development and into late adulthood. Finally, we detail the utilization of this technique in novel astrocyte and astrocyte/neuron co-culture paradigms. © 2019 by John Wiley & Sons, Inc.

Indexed as

AstrocytesMagnetic PhenomenaMicrogliaNeuronsAnimalsBrainCell SeparationFemaleMaleMiceMice, Inbred C57BLastrocytecell isolationco-culturemagnetic cell separationmicroglianeuron

Identifiers

PMID31216394
PMCPMC6653683
OpenAlexW2948599621

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.