ArticlePloS one2019
Aptamer-based fluorometric determination of Salmonella Typhimurium using Fe3O4 magnetic separation and CdTe quantum dots.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Aptamer-Based Label-Free Colorimetric Assay Using Gold Nanoparticles for Specific Detection ofBiosensors · 2026Article
- Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella.Discover nano · 2025Review
- Fluorescent aptasensor for detection of Salmonella typhimurium using boric acid-functionalized terbium metal-organic framework and magnetic nanoparticles.Mikrochimica acta · 2025Article
- Recent Progress in Nanomaterial-Based Fluorescence Assays for the Detection of Food-Borne Pathogens.Sensors (Basel, Switzerland) · 2024Review
- A Systemic Review on Fitness and Survival ofIndian journal of microbiology · 2024Review
- Aptamers: precision tools for diagnosing and treating infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Detection of Emerging Pollutants Using Aptamer-Based Biosensors: Recent Advances, Challenges, and Outlook.Biosensors · 2022Review
- Determination of aflatoxin B1 inRSC advances · 2022Article
- Biosensors, modern technology for the detection of cancer-associated bacteria.Biotechnology letters · 2022Review
- Single-stranded DNA aptamer-based rolling circle amplification as anti-chickenVeterinary world · 2022Article
- Nucleic Acid-Based Nanobiosensor (NAB) Used forNanomaterials (Basel, Switzerland) · 2022Review
- Review
- Review
- Enlarging the Toolbox Against Antimicrobial Resistance: Aptamers and CRISPR-Cas.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on the high sensitivity and stable fluorescence of CdTe quantum dots (QDs) in conjunction with a specific DNA aptamer, the authors describe an aptamer-based fluorescence assay for the determination of Salmonella Typhimurium. The fluorescence detection and quantification of S. Typhimurium is based on a magnetic separation system, a combination of aptamer-coated Fe3O4 magnetic particles (Apt-MNPs) and QD-labeled ssDNA2 (complementary strand of the aptamer). Apt-MNPs are employed for the specific capture of S. Typhimurium. CdTe QD-labeled ssDNA2 was used as a signaling probe. Simply, the as-prepared CdTe QD-labeled ssDNA2 was first incubated with the Apt-MNPs to form the aptamer-ssDNA2 duplex. After the addition of S. Typhimurium, they could specifically bind the DNA aptamer, leading to cleavage of the aptamer-ssDNA2 duplex, accompanied by the release of CdTe QD-labeled DNA. Thus, an increased fluorescence signal can be achieved after magnetic removal of the Apt-MNPs. The fluorescence of CdTe QDs (λexc/em = 327/612 nm) increases linearly in the concentration range of 10 to 1010 cfu•mL-1, and the limit of detection is determined to be 1 cfu•mL-1. The detection process can be performed within 2 h and is successfully applied to the analysis of spiked food samples with good recoveries from 90% to 105%.
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