ArticlePLoS neglected tropical diseases2019
Comparative performance of lateral flow immunochromatography, iELISA and Rose Bengal tests for the diagnosis of cattle, sheep, goat and swine brucellosis.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 53 citations in OpenAlex.
- Epidemiology of Brucellosis Among Deer in China From 1978 to 2025: A Systematic Review and Meta-Analysis.Transboundary and emerging diseases · 2026Pooled it
- Global Comprehensive Literature Review and Meta-Analysis of Brucella spp. in Swine Based on Publications From 2000 to 2020.Frontiers in veterinary science · 2021Pooled it
- Bovine Brucellosis in Dairy Herds in Mali and Niger, 2024: Apparent Seroprevalence and Epidemiological Factors.Pathogens (Basel, Switzerland) · 2026Article
- Prevalence of Brucellosis in Small Ruminants in Africa from 2000 to 2025: A Systematic Review and Meta-Analysis.Veterinary sciences · 2026Review
- IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a fromInternational journal of molecular sciences · 2026Article
- Establishment of a rapid Brucella detection method based on MCDA-CRISPR dual signal amplification system for reducing transfusion-transmitted diseases.Scientific reports · 2026Article
- Serological evaluation of brucellosis in nomadic small ruminants in Southeastern Türkiye.BMC veterinary research · 2026Article
- Optimizing ovine brucellosis serodiagnosis: evaluation of recombinantFrontiers in veterinary science · 2026Article
- To establish an indirect ELISA method for the detection of human brucellosis based on signal peptide-truncated recombinant Cu/Zn SOD and its preliminary clinical verification.Frontiers in cellular and infection microbiology · 2026Article
- Development and evaluation of a novel lateral flow immunoassay for rapid diagnosis of brucellosis across different animal species.Scientific reports · 2025Article
- Preparation of a Brucella multiepitope fusion protein based on bioinformatics and its application in serological diagnosis of human brucellosis.Scientific reports · 2025Article
- Serological, molecular, and clinical diagnosis of caprine brucellosis in Tikrit, Iraq.Open veterinary journal · 2025Article
- Impact of herd mobility on brucellosis seroprevalence and spread risk resulting from cross-border transhumance.Veterinary medicine and science · 2024Article
- Evidence-practice gap analysis in the role of tick in brucellosis transmission: a scoping review.Infectious diseases of poverty · 2024Article
- A review of three decades of use of the cattle brucellosis rough vaccine Brucella abortus RB51: myths and facts.BMC veterinary research · 2023Review
- Evaluation of a Lateral Flow Immunochromatography Assay (LFIA) for Diagnosis and Surveillance of Brucellosis in French Alpine Ibex (Microorganisms · 2023Article
- Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle.Heliyon · 2023Article
- Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep.Veterinary world · 2022Article
- Human and Animal Brucellosis in Nigeria: A Systemic Review and Meta-Analysis in the Last Twenty-One Years (2001-2021).Veterinary sciences · 2022Review
- Facing the Human and Animal Brucellosis Conundrums: The Forgotten Lessons.Microorganisms · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is a world-wide extended zoonosis that causes a grave problem in developing economies. Animal vaccination and diagnosis are essential to control brucellosis, and the need for accurate but also simple and low-cost tests that can be implemented in low-infrastructure laboratories has been emphasized. METHODOLOGY: We evaluated bovine, sheep, goat and swine lateral flow immunochromatography assay kits (LFA), the Rose Bengal test (RBT) and a well-validated protein G indirect ELISA (iELISA) using sera of Brucella culture-positive and unvaccinated brucellosis free livestock. Sera from cattle vaccinated with S19 and RB51 brucellosis vaccines were also tested. Finally, we compared RBT and LFA using sera of white Fulani cattle of unknown bacteriological status from a brucellosis endemic area of Nigeria. RESULTS AND
conclusionsAlthough differences were not statistically significant, RBT showed the highest values for diagnostic sensitivity/specificity in cattle (LFA, 96.6/98.8; RBT, 98.9/100; and iELISA, 96.6/100) and the iELISA yielded highest values in sheep (LFA, 94.0/100; RBT, 92.0/100; iELISA, 100/100), goats (LFA, 95.7/96.2; RBT, 97.8/100; iELISA, 100/100) and pigs (LFA, 92.3/100; RBT, 92.3/100; iELISA, 100/100). Vaccine S19 administered subcutaneously interfered in all tests but conjunctival application minimized the problem. Although designed not to interfere in serodiagnosis, vaccine RB51 interfered in LFA and iELISA but not in the RBT. We found closely similar apparent prevalence results when testing the Nigerian Fulani cattle by RBT and LFA. Although both RBT and LFA (showing similar diagnostic performance) are suitable for small laboratories in resource-limited areas, RBT has the advantage that a single reagent is useful in all animal species. Considering these advantages, its low cost and that it is also useful for human brucellosis diagnosis, RBT might be a good choice for resource-limited laboratories.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.