ArticleRNA biology2019
A two-step probing method to compare lysine accessibility across macromolecular complex conformations.
Andrew J MacRae, Patricia Coltri, Eva Hrabeta-Robinson, Robert J Chalkley, A L Burlingame, Melissa S Jurica
Open access · bronzeAbstract read
In one paragraphArticle in RNA biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
6 authors at 3 institutions in 2 countries.
Andrew J MacRaeDepartment of Molecular, Cell and Developmental Biology, University California , Santa Cruz , USA.ORCID 0000-0002-5676-4312 Patricia ColtriDepartment of Molecular, Cell and Developmental Biology, University California , Santa Cruz , USA.ORCID 0000-0001-8447-7516 Eva Hrabeta-RobinsonDepartment of Molecular, Cell and Developmental Biology, University California , Santa Cruz , USA.
Robert J ChalkleyDepartment of Pharmaceutical Chemistry, University of California San Francisco , San Francisco , CA , USA.
A L BurlingameDepartment of Pharmaceutical Chemistry, University of California San Francisco , San Francisco , CA , USA.
Melissa S JuricaDepartment of Molecular, Cell and Developmental Biology, University California , Santa Cruz , USA.ORCID 0000-0002-4488-1386 University of California, Santa Cruz · USUniversity of California, San Francisco · USUniversidade de São Paulo · BR
Funding
YEAST THREONINE &SERINE KINASESP41RR001614 · NCRR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI JULIUS, DAVID · 1985 to 2011
$24.1MBio-Organic Biomedical Mass Spectromy ResourceP41GM103481 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BURLINGAME, ALMA L · 2012 to 2015
$6.4MTraining Program/Molecular/Cell/Developmental BiologyT32GM008646 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI STROME, SUSAN · 1999 to 2018
$3.7MInterpreting the Structure of the SpliceosomeR01GM072649 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI JURICA, MELISSA S · 2006 to 2016
$2.7MMechanisms of the spliceosome protein SF3B1 and inhibitorsR01GM122279 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI JURICA, MELISSA S · 2017 to 2020
$1.8MPURCHASE OF LTQ FT-ICR HYBRID MASS SPECTROMETER: (HEI) PROTEOMICSS10RR019934 · NCRR · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI BURLINGAME, ALMA L · 2004 to 2004
$1.0MPURCHASE OF MICRO LC/NANO ESIQQTOF MASS SPECTROMETERS10RR015804 · NCRR · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI BURLINGAME, ALMA L · 2001 to 2001
$500kNCRR NIH HHS P41 RR001614NCRR NIH HHS S10 RR015804NCRR NIH HHS S10 RR019934NIGMS NIH HHS P41 GM103481NIGMS NIH HHS R01 GM072649NIGMS NIH HHS R01 GM122279NIGMS NIH HHS T32 GM008646
6 · The paper itselfAbstract
Structural models of large and dynamic molecular complexes are appearing in increasing numbers, in large part because of recent technical advances in cryo-electron microscopy. However, the inherent complexity of such biological assemblies comprising dozens of moving parts often limits the resolution of structural models and leaves the puzzle as to how each functional configuration transitions to the next. Orthogonal biochemical information is crucial to understanding the molecular interactions that drive those rearrangements. We present a two-step method for chemical probing detected by tandem mass-spectrometry to globally assess the reactivity of lysine residues within purified macromolecular complexes. Because lysine side chains often balance the negative charge of RNA in ribonucleoprotein complexes, the method is especially useful for detecting changes in protein-RNA interactions. By probing the
Indexed as
Models, MolecularMolecular ConformationAcetylationCrystallography, X-RayHumansLysineMacromolecular SubstancesPeptidesRibosome Subunits, Small, BacterialRNASpliceosomesTandem Mass SpectrometryLysineMacromolecular SubstancesPeptidesRNAChemical probinglysinemass spectrometryRNA protein complex
Identifiers
PMID31213125
PMCPMC6779377
OpenAlexW2949502218
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