ArticleInternational journal of molecular sciences2019
Melatonin Abrogates the Anti-Developmental Effect of the AKT Inhibitor SH6 in Bovine Oocytes and Embryos.
Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Melatonin During Pre-Maturation and Its Effects on Bovine Oocyte Competence.Antioxidants (Basel, Switzerland) · 2025Article
- Mitochondrial Quality Control in Bovine Oocyte Maturation: Mechanisms, Challenges, and Prospects for Enhancing Reproductive Efficiency.Animals : an open access journal from MDPI · 2025Review
- Effect of Melatonin Supplementation on In Vitro Developmental Competence of Bovine Oocyte: A Systematic Review and Meta-Analysis.Veterinary medicine international · 2025Review
- An Update on Physiopathological Roles of Akt in the ReprodAKTive Mammalian Ovary.Life (Basel, Switzerland) · 2024Review
- Identification of transcriptome characteristics of granulosa cells and the possible role of UBE2C in the pathogenesis of premature ovarian insufficiency.Journal of ovarian research · 2023Article
- Review
- Attenuation of Oxidative Stress and Regulation of AKT Signaling by Vanillic Acid during Bovine Pre-Implantation Embryo Development.Nutrients · 2023Article
- Modulation of Apoptosis and Autophagy by Melatonin in Juglone-Exposed Bovine Oocytes.Animals : an open access journal from MDPI · 2023Article
- Downregulation of PI3K/AKT/mTOR Pathway in Juglone-Treated Bovine Oocytes.Antioxidants (Basel, Switzerland) · 2023Article
- Article
- Exogenous Melatonin in the Culture Medium Does Not Affect the Development of In Vivo-Derived Pig Embryos but Substantially Improves the Quality of In Vitro-Produced Embryos.Antioxidants (Basel, Switzerland) · 2022Article
- Pseudouridylate Synthase 7 Promotes Cell Proliferation and Invasion in Colon Cancer Through Activating PI3K/AKT/mTOR Signaling Pathway.Digestive diseases and sciences · 2022Article
- The Role of MAPK3/1 and AKT in the Acquisition of High Meiotic and Developmental Competence of Porcine Oocytes Cultured In Vitro in FLI Medium.International journal of molecular sciences · 2021Article
- Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress.Bioscience reports · 2021Article
- Melatonin: Multi-Target Mechanism Against Diminished Ovarian Reserve Based on Network Pharmacology.Frontiers in endocrinology · 2021Article
- Procyanidin B1 promotes in vitro maturation of pig oocytes by reducing oxidative stress.Molecular reproduction and development · 2021Article
- Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling.Oxidative medicine and cellular longevity · 2021Article
- Applications of Melatonin in Female Reproduction in the Context of Oxidative Stress.Oxidative medicine and cellular longevity · 2021Review
- Induction of Oxidative Stress and Mitochondrial Dysfunction by Juglone Affects the Development of Bovine Oocytes.International journal of molecular sciences · 2020Article
- Coping with DNA Double-Strand Breaks via ATM Signaling Pathway in Bovine Oocytes.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Melatonin, a nighttime-secreted antioxidant hormone produced by the pineal gland, and AKT, a serine/threonine-specific protein kinase, have been identified as regulators for several cellular processes essential for reproduction. The current study aimed to investigate the potential interplay between melatonin and AKT in bovine oocytes in the context of embryo development. Results showed that the inclusion of SH6, a specific AKT inhibitor, during in vitro maturation (IVM) significantly reduced oocyte maturation, cumulus cell expansion, cleavage, and blastocyst development that were rescued upon addition of melatonin. Oocytes treated with SH6 in the presence of melatonin showed lower levels of reactive oxygen species (ROS) and blastocysts developed exhibited low apoptosis while the mitochondrial profile was significantly improved compared to the SH6-treated group. The RT-qPCR results showed up-regulation of the mRNA of maturation-, mitochondrial-, and cumulus expansion-related genes including GDF-9, BMP-15, MARF1, ATPase, ATP5F1E, POLG2, HAS2, TNFAIP6, and PTGS2 and down-regulation of Bcl-2 associated X apoptosis regulator (BAX), caspase 3, and p21 involved in apoptosis and cell cycle arrest in melatonin-SH6 co-treated group compared to SH6 sole treatment. The immunofluorescence showed high levels of caspase 3 and caspase 9, and low AKT phosphorylation in the SH6-treated group compared to the control and melatonin-SH6 co-treatment. Taken together, our results showed the importance of both melatonin and AKT for overall embryonic developmental processes and, for the first time, we report that melatonin could neutralize the deleterious consequences of AKT inhibition, suggesting a potential role in regulation of AKT signaling in bovine oocytes.
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Registered trials
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