ArticleMolecular human reproduction2019
Human blastocyst outgrowths recapitulate primordial germ cell specification events.
Article in Molecular human reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Reconstitution of the cellular niche requirements for primordial germ cell-like cell progression in humans.Stem cell reports · 2026Article
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026Review
- Extended In Vitro Culture of Human Embryos.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025Review
- The establishment and regulation of human germ cell lineage.Stem cell research & therapy · 2025Review
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- Early human development and stem cell-based human embryo models.Cell stem cell · 2024Review
- Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Stay on the road: from germ cell specification to gonadal colonization in mammals.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Gastruloids: Pluripotent stem cell models of mammalian gastrulation and embryo engineering.Developmental biology · 2022Article
- Trophectoderm differentiation to invasive syncytiotrophoblast is promoted by endometrial epithelial cells during human embryo implantation.Human reproduction (Oxford, England) · 2022Article
- Establishment, characterization, and validation of novel porcine embryonic fibroblasts as a potential source for genetic modification.Frontiers in cell and developmental biology · 2022Article
- Article
- Engineered models of the human embryo.Nature biotechnology · 2021Article
- Sex-Specific Isolation and Propagation of Human Premeiotic Fetal Germ Cells and Germ Cell-Like Cells.Cells · 2021Article
- Activin A-derived human embryonic stem cells show increased competence to differentiate into primordial germ cell-like cells.Stem cells (Dayton, Ohio) · 2021Article
- Mammalian primordial germ cell specification.Development (Cambridge, England) · 2021Review
- Ligand-Receptor Interactions Elucidate Sex-Specific Pathways in the Trajectory From Primordial Germ Cells to Gonia During Human Development.Frontiers in cell and developmental biology · 2021Article
- Human Primordial Germ Cells Are Specified from Lineage-Primed Progenitors.Cell reports · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our current knowledge of the mechanisms leading to human primordial germ cell (PGC) specification stems solely from differentiation experiments starting from human pluripotent stem cells. However, information regarding the origin of PGCs in vivo remains obscure. Here we apply an improved system for extended in vitro culture of human embryos to investigate the presence of PGC-like cells (PGCLCs) 12 days post fertilization (dpf). Good quality blastocysts (n = 141) were plated at 6 dpf and maintained in hypoxia, in medium supplemented with Activin A until 12 dpf. We primarily reveal that 12 dpf outgrowths recapitulate human peri-implantation events and demonstrate that blastocyst quality significantly impacts both embryo viability at 12 dpf, as well as the presence of POU5F1+ cells within viable outgrowths. Moreover, detailed examination of 12 dpf blastocyst outgrowths revealed a population of POU5F1+, SOX2- and SOX17+ cells that may correspond to PGCLCs, alongside POU5F1+ epiblast-like cells and GATA6+ endoderm-like cells. Our findings suggest that, in human, PGC precursors may become specified within the epiblast and migrate either transiently to the extra-embryonic mesoderm or directly to the dorsal part of the yolk sac endoderm around 12 dpf. This is a descriptive analysis and as such the conclusion that POU5F1+ and SOX17+ cells represent bona fide PGCs can only be considered as preliminary. In the future, other PGC markers may be used to further validate the observed cell populations. Overall, our findings provide insights into the origin of the human germline and may serve as a foundation to further unravel the molecular mechanisms governing PGC specification in human.
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