ArticleScientific reports2019
Depletion of Intestinal Microbiome Partially Rescues Bone Loss in Sickle Cell Disease Male Mice.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- A mouse model for hemoglobin SC disease recapitulates characteristic human pathologies.Blood advances · 2025Article
- Hemoglobin S Promotes Glycemic Dysregulation in a Mouse Model of Human Sickle Cell Disease.Endocrinology · 2025Article
- Fibroblast growth factor 23 neutralizing antibody partially rescues bone loss and increases hematocrit in sickle cell disease mice.Scientific reports · 2025Article
- MyD88 in osteoclast and osteoblast lineages differentially controls bone remodeling in homeostasis and malaria.International immunology · 2024Article
- From gut to bone: deciphering the impact of gut microbiota on osteoporosis pathogenesis and management.Frontiers in cellular and infection microbiology · 2024Review
- Gut microbiota signatures and fecal metabolites in postmenopausal women with osteoporosis.Gut pathogens · 2023Article
- Intestinal barrier dysfunction in murine sickle cell disease is associated with small intestine neutrophilic inflammation, oxidative stress, and dysbiosis.FASEB bioAdvances · 2023Article
- Intestinal barrier functions in hematologic and oncologic diseases.Journal of translational medicine · 2023Review
- Characterization of Fecal Microbiomes of Osteoporotic Patients in Korea.Polish journal of microbiology · 2022Article
- Bone loss is ameliorated by fecal microbiota transplantation through SCFA/GPR41/ IGF1 pathway in sickle cell disease mice.Scientific reports · 2022Article
- Microbial gut evaluation in an angolan paediatric population with sickle cell disease.Journal of cellular and molecular medicine · 2022Article
- The Role of Depletion of Gut Microbiota in Osteoporosis and Osteoarthritis: A Narrative Review.Frontiers in endocrinology · 2022Review
- A gene co-association network regulating gut microbial communities in a Duroc pig population.Microbiome · 2021Article
- The role of gut microbiota in bone homeostasis.Bone & joint research · 2021Article
- Gut microbiome and osteoporosis: a review.Bone & joint research · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteoporosis or osteopenia are common clinical manifestations of sickle cell disease (SCD) with unclear mechanisms. Since senescence of circulating neutrophil can be modulated by signals derived from intestinal microbiome and neutrophils are abundant in bone marrow and can regulate osteoblasts and osteoclasts, we examined whether gut microbiome contributes to bone loss in SCD mice. SCD and their littermates control mice were treated with antibiotics to deplete gut microbiome. At the end of 7 weeks treatment, serum was collected for biochemistry marker measurements. Bone mass and remodeling were evaluated by dual beam X-ray absorptiometry, micro-computed tomography, and histomorphometry. Bone-related genes in tibia and barrier marker genes in the small intestine were analyzed by quantitative PCR. Antibiotic treatment rescued increased intestinal inflammatory cytokine marker genes (Tnfα, IL17, Ifnγ) expression, rescued decreased intestinal barrier marker genes (claudin 3 and claudin 15) expression, and rescued increased serum cytokines (IFNγ, IL27, IL10) in SCD mice. Antibiotic significantly improved decreased bone mass in SCD mice mainly through enhanced osteoblast function and increased osteoblast-related genes (Runx2 and Igf1) expression in SCD mice. Our findings support that increased bacteria load augments antigenic load traversing the impaired intestinal barrier through inflammation, leading to increased inflammatory cytokines, impaired osteoblast function, and bone loss in SCD mice.
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