ArticleScientific reports2019
RNA interference core components identified and characterised in Verticillium nonalfalfae, a vascular wilt pathogenic plant fungi of hops.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 24 citations in OpenAlex.
- Tracking of Tobacco Mosaic Virus in Taxonomically Different Plant Fungi.Journal of fungi (Basel, Switzerland) · 2025Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Direct Molecular Evidence for an Ancient, Conserved Developmental Toolkit Controlling Posttranscriptional Gene Regulation in Land Plants.Molecular biology and evolution · 2021Article
- Recent Approaches towards Control of Fungal Diseases in Plants: An Updated Review.Journal of fungi (Basel, Switzerland) · 2021Review
- Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants.International journal of molecular sciences · 2021Review
- Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging withInternational journal of molecular sciences · 2021Article
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Repetitive Elements Contribute to the Diversity and Evolution of Centromeres in the Fungal GenusmBio · 2020Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The conserved RNA interference mechanism (RNAi) in the fungal kingdom has become a focus of intense scientific investigation. The three catalytic core components, Dicer-like (DCL), Argonaute (AGO), and RNA-dependent RNA polymerase (RdRP), and their associated small interfering RNA molecules (siRNAs) have been identified and characterised in several fungal species. Recent studies have proposed that RNAi is a major contributor to the virulence of fungal pathogens as a result of so-called trans-kingdom RNA silencing. In the present study, we report on the existence of three core RNAi proteins in the pathogenic plant fungus Verticillium nonalfalfae, which is a soilborne plant pathogen that causes severe wilting disease in hops (Humulus lupulus L.). Two DCL proteins, two AGO proteins, and two RdRP proteins were identified, and their conserved RNAi domains were characterised. Our phylogeny results confirm the existing taxonomic relationships in the Ascomycete fungal phylum and show that the fungi of the Hypocreomycetidae subclass of the Sordariomycetes class have high amino acid sequence similarity. The expression analysis revealed a potential role of RNAi in the pathogenicity of the fungi, since all the RNAi genes were highly upregulated in the highly virulent isolate T2 and were also differentially expressed in the V. nonalfalfae-susceptible Celeia and V. nonalfalfae-resistant Wye Target cultivars.
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