Evidence map›Paper›PMID 31209205›Full record

ArticleCell death & disease2019

miR-381 modulates human bone mesenchymal stromal cells (BMSCs) osteogenesis via suppressing Wnt signaling pathway during atrophic nonunion development.

Haitao Long, Yong Zhu, Zhangyuan Lin, Jun Wan, Liang Cheng, Min Zeng, Yifu Tang, Ruibo Zhao

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. Pooled it
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  19. MicroRNA-loaded biomaterials for osteogenesis.Frontiers in bioengineering and biotechnology · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Haitao LongDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.ORCID http://orcid.org/0000-0001-5084-8569
Yong ZhuDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Zhangyuan LinDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Jun WanDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Liang ChengDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Min ZengDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Yifu TangDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China.
Ruibo ZhaoDepartment of Orthopaedics, Xiangya Hospital, Central South University, Changsha, 410008, China. drzhaoruibo@163.com.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The osteogenic differentiation of human bone mesenchymal stromal cells (BMSCs) has been considered as a central issue in fracture healing. Wnt signaling could promote BMSC osteogenic differentiation through inhibiting PPARγ. During atrophic nonunion, Wnt signaling-related factors, WNT5A and FZD3 proteins, were significantly reduced, along with downregulation of Runx2, ALP, and Collagen I and upregulation of PPARγ. Here, we performed a microarray analysis to identify differentially expressed miRNAs in atrophic nonunion tissues that were associated with Wnt signaling through targeting related factors. Of upregulated miRNAs, miR-381 overexpression could significantly inhibit the osteogenic differentiation in primary human BMSCs while increase in PPARγ protein level. Through binding to the 3'UTR of WNT5A and FZD3, miR-381 modulated the osteogenic differentiation via regulating β-catenin nucleus translocation. Moreover, PPARγ, an essential transcription factor inhibiting osteogenic differentiation, could bind to the promoter region of miR-381 to activate its expression. Taken together, PPARγ-induced miR-381 upregulation inhibits the osteogenic differentiation in human BMSCs through miR-381 downstream targets, WNT5A and FZD3, and β-catenin nucleus translocation in Wnt signaling. The in vivo study also proved that inhibition of miR-381 promoted the fracture healing. Our finding may provide a novel direction for atrophic nonunion treatment.

Indexed as

OsteogenesisWnt Signaling PathwayAnimalsAtrophyBase Sequencebeta CateninCell DifferentiationCell NucleusCells, CulturedDown-RegulationFemoral FracturesFracture HealingFractures, UnunitedHumansMaleMesenchymal Stem Cellsbeta CateninMicroRNAsMIRN381 microRNA, humanPPAR gamma

Identifiers

PMID31209205
PMCPMC6572824
OpenAlexW2950874447

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.