ArticleProceedings of the National Academy of Sciences of the United States of America2019
Compartmentalized biosynthesis of mycophenolic acid.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 84 citations in OpenAlex.
- Novel production of vinblastine by immobilized cultures of mutant strain of the endophytic Alternaria alternata.World journal of microbiology & biotechnology · 2026Article
- Six Undescribed Lanostane-Type Triterpenoids From Ganoderma lucidum.Chemistry & biodiversity · 2026Article
- Nitrile rubber biodegradation byApplied and environmental microbiology · 2025Article
- Enhancing the β-Oxidation-Like Pathway for the Optimal Production of the Immunosuppressant Mycophenolic Acid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Tracing the Origin and Evolution of the Fungal Mycophenolic Acid Biosynthesis Pathway.Genome biology and evolution · 2025Article
- Involvement of a Noncanonical Polyketide Synthase-Nonribosomal Peptide Synthetase Hybrid in the Biosynthesis of Sterol-C4-methyl Oxidase Inhibitor PF1163A.Organic letters · 2025Article
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Early Steps of the Biosynthesis of the Anticancer Antibiotic Pleurotin.ACS chemical biology · 2024Article
- Structural basis for substrate flexibility of the O-methyltransferase MpaG' involved in mycophenolic acid biosynthesis.Protein science : a publication of the Protein Society · 2024Article
- Heterologous expression facilitates the discovery and characterization of marine microbial natural products.Engineering microbiology · 2024Review
- Exploring the Subcellular Localization ofJournal of fungi (Basel, Switzerland) · 2024Article
- Activation of secondary metabolite gene clusters in Chaetomium olivaceum via the deletion of a histone deacetylase.Applied microbiology and biotechnology · 2024Article
- Article
- Targeting of Specialized Metabolites Biosynthetic Enzymes to Membranes and Vesicles by Posttranslational Palmitoylation: A Mechanism of Non-Conventional Traffic and Secretion of Fungal Metabolites.International journal of molecular sciences · 2024Review
- Production of non-natural 5-methylorsellinate-derived meroterpenoids inBeilstein journal of organic chemistry · 2024Article
- Engineered and total biosynthesis of fungal specialized metabolites.Nature reviews. Chemistry · 2024Review
- Secondary Metabolites Produced by the Blue-Cheese Ripening MoldJournal of fungi (Basel, Switzerland) · 2023Review
- High-efficient production of mushroom polyketide compounds in a platform host Aspergillus oryzae.Microbial cell factories · 2023Article
- Orthoester formation in fungal meroterpenoid austalide F biosynthesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
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Authors and funding
17 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycophenolic acid (MPA) from filamentous fungi is the first natural product antibiotic to be isolated and crystallized, and a first-line immunosuppressive drug for organ transplantations and autoimmune diseases. However, some key biosynthetic mechanisms of such an old and important molecule have remained unclear. Here, we elucidate the MPA biosynthetic pathway that features both compartmentalized enzymatic steps and unique cooperation between biosynthetic and β-oxidation catabolism machineries based on targeted gene inactivation, feeding experiments in heterologous expression hosts, enzyme functional characterization and kinetic analysis, and microscopic observation of protein subcellular localization. Besides identification of the oxygenase MpaB' as the long-sought key enzyme responsible for the oxidative cleavage of the farnesyl side chain, we reveal the intriguing pattern of compartmentalization for the MPA biosynthetic enzymes, including the cytosolic polyketide synthase MpaC' and
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.