ArticleBMC genomics2019
Isolated C. elegans germ nuclei exhibit distinct genomic profiles of histone modification and gene expression.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- RNA Pol I shapes meiotic chromatin, germline H3K4me3 dynamics, and oogenesis independent of ribosome assembly.Cell reports · 2026Article
- Temporal transcriptomic profiling reveals distinct age-associated gene expression signatures in gonads under reduced insulin/IGF-1 signaling in Caenorhabditis elegans.Cell communication and signaling : CCS · 2025Article
- RNA Pol I activity is required for meiotic chromatin organization and the H3K4me3 gradient essential for oogenesis, independent of ribosome synthesis.bioRxiv : the preprint server for biology · 2025Article
- Regulation of MORC-1 is key to the CSR-1-mediated germline gene licensing mechanism inScience advances · 2025Article
- Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.Cell discovery · 2025Article
- FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.Development (Cambridge, England) · 2025Article
- Crossovers are regulated by a conserved and disordered synaptonemal complex domain.Nucleic acids research · 2025Article
- Non-cell-autonomous regulation of germline proteostasis by insulin/IGF-1 signaling-induced dietary peptide uptake via PEPT-1.The EMBO journal · 2024Article
- The Upstream Sequence Transcription Complex dictates nucleosome positioning and promoter accessibility at piRNA genes in the C. elegans germ line.PLoS genetics · 2024Article
- Skp1 proteins are structural components of the synaptonemal complex inScience advances · 2024Article
- Characterization of the distribution and dynamics of chromatin states in theGenome research · 2024Article
- HRDE-2 drives small RNA specificity for the nuclear Argonaute protein HRDE-1.Nature communications · 2024Article
- SIN-3 acts in distinct complexes to regulate the germline transcriptional program in Caenorhabditis elegans.Development (Cambridge, England) · 2023Article
- A cooperative network at the nuclear envelope counteracts LINC-mediated forces during oogenesis inScience advances · 2023Article
- Review
- Bergerac strains of Caenorhabditis elegans revisited: expansion of Tc1 elements imposes a significant genomic and fitness cost.G3 (Bethesda, Md.) · 2022Article
- Aging and sperm signals alter DNA break formation and repair in the C. elegans germline.PLoS genetics · 2022Article
- Article
- Chromatin accessibility profiling by ATAC-seq.Nature protocols · 2022Review
- Laser Microdissection for Species-Agnostic Single-Tissue Applications.Journal of visualized experiments : JoVE · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe wide variety of specialized permissive and repressive mechanisms by which germ cells regulate developmental gene expression are not well understood genome-wide. Isolation of germ cells with high integrity and purity from living animals is necessary to address these open questions, but no straightforward methods are currently available.
resultsHere we present an experimental paradigm that permits the isolation of nuclei from C. elegans germ cells at quantities sufficient for genomic analyses. We demonstrate that these nuclei represent a very pure population and are suitable for both transcriptome analysis (RNA-seq) and chromatin immunoprecipitation (ChIP-seq) of histone modifications. From these data, we find unexpected germline- and soma-specific patterns of gene regulation.
conclusionsThis new capacity removes a major barrier in the field to dissect gene expression mechanisms in the germ line of C. elegans. Consequent discoveries using this technology will be relevant to conserved regulatory mechanisms across species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.