Evidence map›Paper›PMID 31206838›Full record

ArticleCell proliferation2019

Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask.

Huimin He, Qing He, Feiyue Xu, Yan Zhou, Zhaoyang Ye, Wen-Song Tan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 35 citations in OpenAlex.

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  16. Fabrication of PNIPAm-based thermoresponsive hydrogel microwell arrays for tumor spheroid formation.Materials science & engineering. C, Materials for biological applications · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Huimin HeThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Qing HeThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Feiyue XuThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yan ZhouThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Zhaoyang YeThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID https://orcid.org/0000-0001-5136-2384
Wen-Song TanThe State Kay Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
East China University of Science and Technology · CN

Funding

Basic Research Key Program Project of Commission of Science and Technology of Shanghai 16JC1400203National Key Research and Development Program of China 2018YFC1105801National Natural Science Foundation of China 31170951National Natural Science Foundation of China 81671841Natural Science Foundation of Shanghai 16ZR1408700
6 · The paper itself

Abstract

objectivesCellular aggregates are readily applicable in cell-based therapy. The effects of agitation and inoculation density on the aggregation of cells in spinner flask and the molecular mechanism of aggregation were investigated. MATERIALS AND

methodsThe aggregation kinetics of cells in spinner flask was evaluated with bovine articular chondrocytes (bACs), rabbit bone marrow-derived mesenchymal stem cells (rMSCs) and their mixture. The morphology of cellular aggregates was studied with scanning electron microscopy and gene expression of cell adhesion-related molecules was analysed.

resultsIt was shown that suspension culture in spinner flask induced the aggregation of bACs and rMSCs. Both cells exhibited increased aggregation rate and aggregate size with decreasing agitation rate and increasing cell inoculation density. Additionally, aggregate size increased with extended culture time. By analysing gene expression of integrin β1 and cadherin, it was indicated that these molecules were potentially involved in the aggregation process of bACs and rMSCs, respectively. Aggregates composed of both bACs and rMSCs were also prepared, showing rMSCs in the core and bACs in the periphery.

conclusionsCellular aggregates were prepared in dynamic suspension culture using spinner flask, the key parameters to the aggregation process were identified, and the molecular mechanism of aggregation was revealed. This would lay a solid foundation for the large-scale production of cellular aggregates for cell-based therapy, such as cartilage regeneration.

Indexed as

AnimalsBone MarrowCattleCell AdhesionCell Adhesion MoleculesCell- and Tissue-Based TherapyCell CountCell Culture TechniquesCells, CulturedChondrocytesGene ExpressionMesenchymal Stem CellsRabbitsCell Adhesion Moleculesaggregation kineticsarticular chondrocytescell aggregationmesenchymal stem cellsspinner flask

Identifiers

PMID31206838
PMCPMC6669002
OpenAlexW2951502469

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.