ArticleElectrophoresis2019
Mathematical model to reduce loop mediated isothermal amplification (LAMP) false-positive diagnosis.
Article in Electrophoresis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Ultrafast visual detection of Streptococcus suis via HtpsC-targeted LAMP-CRISPR-Cas12a coupled with lateral flow immunochromatography.FEBS open bio · 2026Article
- Advancements and Emerging Trends in the Detection of Sweet Potato Diseases: A Comprehensive Review.ACS omega · 2026Review
- Sensitive and rapid detection ofFrontiers in cellular and infection microbiology · 2026Article
- A point-of-care testing device for monkeypox virus integrating recombinase polymerase amplification and lateral flow assay.BMC medicine · 2025Article
- LAMP-Based 4-Channel Microfluidic Chip for POCT Detection of Influenza A H1N1, H3N2, and Influenza B Victoria Viruses.Biosensors · 2025Article
- Mpox disease, diagnosis, and point of care platforms.Bioengineering & translational medicine · 2025Review
- A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents:International journal of molecular sciences · 2024Article
- Article
- Establishment and application of a loop-mediated isothermal amplification-lateral flow dipstick (LAMP-LFD) method for detecting Clostridium piliforme.Veterinary medicine and science · 2024Article
- Development and evaluation of rapid and simple detection ofFrontiers in microbiology · 2024Article
- Manually pressurized droplet digital PCR chip for rapid SARS-CoV-2 diagnostics.Biomicrofluidics · 2024Article
- Article
- Data treatment methods for real-time colorimetric loop-mediated isothermal amplification reactions.Scientific reports · 2023Article
- Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.3 Biotech · 2023Review
- CDetection.v2: One-pot assay for the detection of SARS-CoV-2.Frontiers in microbiology · 2023Article
- Loop-mediated isothermal amplification (LAMP)/Cas12a assay for detection ofFrontiers in bioengineering and biotechnology · 2023Article
- Cas12a-assisted RTF-EXPAR for accurate, rapid and simple detection of SARS-CoV-2 RNA.Biosensors & bioelectronics · 2022Article
- Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction.The Analyst · 2022Article
- Review
- Argonaute-integrated isothermal amplification for rapid, portable, multiplex detection of SARS-CoV-2 and influenza viruses.Biosensors & bioelectronics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Loop mediated isothermal amplification (LAMP) is a nucleic acid amplification technique performed under isothermal conditions. The output of this amplification technique includes multiple different sizes of deoxyribonucleic acid (DNA) structures which are identified by a banding pattern on gel electrophoresis plots. Although this is a specific amplification technique, the complexity of the primer design and amplification still lead to the issue of obtaining false-positive results, especially when a positive reading is determined solely by whether there is any banding pattern in the gel electrophoresis plot. Here, we first performed extensive LAMP experiments and evaluated the DNA structures using microchip electrophoresis. We then developed a mathematical model derived from the various components that make up an entire LAMP structure to predict the full LAMP structure size in base pairs. This model can be implemented by users to make predictions for specific, DNA size dependent, banding patterns on their gel electrophoresis plots. Each prediction is specific to the target sequence and primers used and therefore reduces incorrect diagnosis errors through identifying true-positive and false-positive results. This model was accurately tested with multiple primer sets in house and was also translatable to different DNA and RNA types in previously published literature. The mathematical model can ultimately be used to reduce false-positive LAMP diagnosis errors for applications ranging from tuberculosis diagnostics to E. coli to numerous other infectious diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.