ArticleJournal of tissue engineering
Optimizing growth factor induction of tenogenesis in three-dimensional culture of mesenchymal stem cells.
Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
19 citing papers in PubMed.
- The Characterization of Serum-Free Media on Human Mesenchymal Stem Cell Fibrochondrogenesis.Bioengineering (Basel, Switzerland) · 2025Article
- Review
- Augmentation of Tendon and Ligament Repair with Fiber-Reinforced Hydrogel Composites.Advanced healthcare materials · 2024Review
- Engineered Microenvironmental Cues from Fiber-Reinforced Hydrogel Composites Drive Tenogenesis and Aligned Collagen Deposition.Advanced healthcare materials · 2024Article
- In Vitro and In Vivo Effects of IGF-1 Delivery Strategies on Tendon Healing: A Review.International journal of molecular sciences · 2023Review
- Wetspun Polymeric Fibrous Systems as Potential Scaffolds for Tendon and Ligament Repair, Healing and Regeneration.Pharmaceutics · 2022Review
- Advanced Nanofiber-Based Scaffolds for Achilles Tendon Regenerative Engineering.Frontiers in bioengineering and biotechnology · 2022Review
- Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture.Nature communications · 2021Article
- Proliferation and tenogenic differentiation of bone marrow mesenchymal stem cells in a porous collagen sponge scaffold.World journal of stem cells · 2021Article
- Basic Research on Tendon Repair: Strategies, Evaluation, and Development.Frontiers in medicine · 2021Review
- A brief history of tendon and ligament bioreactors: Impact and future prospects.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Review
- In Vitro Innovation of Tendon Tissue Engineering Strategies.International journal of molecular sciences · 2020Review
- Induction of tenogenic differentiation of equine adipose-derived mesenchymal stem cells by platelet-derived growth factor-BB and growth differentiation factor-6.Molecular biology reports · 2020Article
- Concurrent multi-lineage differentiation of mesenchymal stem cells through spatial presentation of growth factors.Biomedical materials (Bristol, England) · 2020Article
- Multi-differentiation potential is necessary for optimal tenogenesis of tendon stem cells.Stem cell research & therapy · 2020Article
- From the perspective of embryonic tendon development: various cells applied to tendon tissue engineering.Annals of translational medicine · 2020Review
- Hypoxia-Induced Mesenchymal Stem Cells Exhibit Stronger Tenogenic Differentiation Capacities and Promote Patellar Tendon Repair in Rabbits.Stem cells international · 2020Article
- Effect of Acellular Amnion With Increased TGF-β and bFGF Levels on the Biological Behavior of Tenocytes.Frontiers in bioengineering and biotechnology · 2020Article
- The glucagon-like peptide 1 receptor agonist Exendin-4 induces tenogenesis in human mesenchymal stem cells.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adult tissue stem cells have shown promise for the treatment of debilitating tendon injuries. However, few comparisons of stem cells from different tissue sources have been made to determine the optimum stem cell source for treating tendon. Moreover, it is likely that the application of tenogenic growth factors will improve tendon stem cell treatments further, and a comprehensive comparison of a number of growth factors is needed. Thus far, different types of stem cells cannot be evaluated in a high-throughput manner. To this end, we have developed an approach to culture mesenchymal stem cells isolated from bone marrow in collagen type I hydrogels with tenogenic growth factors using economical, commercially available supplies. To optimize growth factors for this assay, FGF-2, TGF-β1, IGF-1, and/or BMP-12 were tested singly and in novel combinations of (1) BMP-12 and IGF-1, (2) TGF-β1 and IGF-1, and/or (3) BMP-12 and FGF-2 over 10 days. Our data suggest that BMP-12 supplementation alone results in the strongest expression of tendon marker genes, controlled contractility of constructs, a higher degree of cell alignment, and tendon-like tissue morphology. This easy-to-use benchtop assay can be used to screen novel sources of stem cells and cell lines for tissue engineering and tendon healing applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.