Evidence map›Paper›PMID 31202698›Full record

ArticleJournal of plastic, reconstructive & aesthetic surgery : JPRAS2020

Adhesion, distribution, and migration of differentiated and undifferentiated mesenchymal stem cells (MSCs) seeded on nerve allografts.

Femke Mathot, Nadia Rbia, Allen T Bishop, Steven E R Hovius, Andre J Van Wijnen, Alexander Y Shin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.1field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
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  9. Introducing human adipose-derived mesenchymal stem cells to AvanceJournal of plastic, reconstructive & aesthetic surgery : JPRAS · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Femke MathotDepartment of Orthopedic Surgery, Division of Hand and Microvascular Surgery, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA; Department of Plastic Surgery, Radboudumc, Geert Grooteplein Zuid 10, 6525GA Nijmegen, the Netherlands.
Nadia RbiaDepartment of Orthopedic Surgery, Division of Hand and Microvascular Surgery, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA.
Allen T BishopDepartment of Orthopedic Surgery, Division of Hand and Microvascular Surgery, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA.
Steven E R HoviusDepartment of Plastic Surgery, Radboudumc, Geert Grooteplein Zuid 10, 6525GA Nijmegen, the Netherlands; Hand and Wrist Surgery, Xpert Clinic, Jan Leentvaarlaan 14-24, 3065 DC Rotterdam, the Netherlands.
Andre J Van WijnenDepartment of Orthopedic Surgery, Division of Hand and Microvascular Surgery, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA.
Alexander Y ShinDepartment of Orthopedic Surgery, Division of Hand and Microvascular Surgery, Mayo Clinic, 200 First Street S.W., Rochester, MN 55905, USA. Electronic address: shin.alexander@mayo.edu.
Mayo Clinic · USRadboud University Nijmegen · NL

Funding

Bridging the gap: Angiogenesis and stem cell seeding of processed nerve allograftR01NS102360 · NINDS · MAYO CLINIC ROCHESTER · PI SHIN, ALEXANDER Y. · 2018 to 2022
$1.7M
NINDS NIH HHS R01 NS102360
6 · The paper itself

Abstract

backgroundAlthough undifferentiated MSCs and MSCs differentiated into Schwann-like cells have been extensively compared in vitro and in vivo, studies on the ability and efficiency of differentiated MSCs for delivery into nerve allografts are lacking. As this is essential for their clinical potential, the purpose of this study was to determine the ability of MSCs differentiated into Schwann-like cells to be dynamically seeded on decellularized nerve allografts and to compare their seeding potential to that of undifferentiated MSCs.

methodsFifty-six sciatic nerve segments from Sprague Dawley rats were decellularized, and MSCs were harvested from Lewis rat adipose tissue. Control and differentiated MSCs were dynamically seeded on the surface of decellularized allografts. Cell viability, seeding efficiencies, cell adhesion, distribution, and migration were evaluated.

resultsThe viability of both cell types was not influenced by the processed nerve allograft. Both cell types achieved maximal seeding efficiency after 12 h of dynamic seeding, albeit that differentiated MSCs had a significantly higher mean seeding efficiency than control MSCs. Dynamic seeding resulted in a uniform distribution of cells among the surface of the nerve allograft. No cells were located inside the nerve allograft after seeding.

conclusionDifferentiated MSCs can be dynamically seeded on the surface of a processed nerve allograft, in a similar fashion as undifferentiated MSCs. Schwann-like differentiated MSCs have a significantly higher seeding efficiency after 12 h of dynamic seeding. We conclude that differentiation of MSCs into Schwann-like cells may improve the seeding strategy and the ability of nerve allografts to support axon regeneration.

Indexed as

AllograftsAnimalsCell AdhesionCell MovementCell SurvivalMesenchymal Stem CellsMesenchymal Stem Cell TransplantationNerve RegenerationRats, Inbred LewRats, Sprague-DawleySchwann CellsSciatic NerveTransplantation, HomologousDifferentiationMSCsProcessed nerve allograftSchwann-like cellsSeeding

Identifiers

PMID31202698
PMCPMC6872919
OpenAlexW2946492914

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.