Evidence map›Paper›PMID 31202581›Full record

ArticleEuropean journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery2019

Single Muscle Fibre Contractility Testing in Rats to Quantify Ischaemic Muscle Damage During Reperfusion Injury.

Emre Gok, Fares Alghanem, Eunice Lim, Dylan Sarver, Logan Eckhardt, Shelby Christensen, Mark Hoenerhoff, Christopher Mendias, Kagan Ozer

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Emre GokDepartment of Surgery, University of Michigan Health System, Ann Arbor, MI, USA; Department of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Fares AlghanemDepartment of Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Eunice LimDepartment of Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Dylan SarverDepartment of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Logan EckhardtDepartment of Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Shelby ChristensenDepartment of Surgery, University of Michigan Health System, Ann Arbor, MI, USA.
Mark HoenerhoffIn Vivo Animal Core, Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI, USA.
Christopher MendiasDepartment of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI, USA; Hospital for Special Surgery, New York, NY, USA.
Kagan OzerDepartment of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI, USA. Electronic address: kozer@med.umich.edu.
University of Michigan · USMichigan Medicine · USHospital for Special Surgery · US

Funding

Structure, Composition, & Histology Core - Core BP30AR069620 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KARL J JEPSEN · 2016 to 2026
$8.4M
NIAMS NIH HHS P30 AR069620
6 · The paper itself

Abstract

objectivesIn this study, the aim was to investigate the potential for single muscle fibre contractility (SMFC) testing to detect the extent of reperfusion injury following various reperfusion periods. The hypothesis was that force generated by muscle fibres will correlate inversely with the extent of reperfusion injury.

methodsTwenty-four Lewis rats were distributed among five groups. Group 1 served as normal muscle control. In all other groups, femoral artery flow was occluded for four hours. Muscle biopsies were obtained at 0 hour, six hours, day two, and day seven after reperfusion in Groups 2, 3, 4, and 5, respectively. Samples then underwent ultrastructural analysis (H&E stain) and SMFC testing.

resultsThe maximum isometric force (mN) generated on Days two and seven after reperfusion decreased from baseline by 21% (p < 0.05), and 53% (p < .001), respectively. The specific force (kPa) followed a similar pattern with a 13% decrease at Day two (p > 0.05) and 31% decrease at Day 7 (p < .001). These results correlated inversely with the extent of quantitative injury on histology.

conclusionsThe study demonstrated an inverse relationship between single muscle fibre contractility testing and neutrophil infiltration during the reperfusion phase. Further clinical studies are needed to evaluate its potential in providing prognostic information for patient outcomes.

Indexed as

Muscle ContractionAnimalsBiopsyConstrictionDisease Models, AnimalFemaleFemoral ArteryHindlimbMuscle Fibers, SkeletalMuscle, SkeletalMuscle StrengthNeutrophil InfiltrationPredictive Value of TestsRats, Inbred LewRegional Blood FlowReperfusion InjuryIschemia–reperfusion injuryMuscleSingle muscle fibre contractility

Identifiers

PMID31202581
PMCPMC12875428
OpenAlexW2950913628

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.