ArticleCell research2019
NDP52 tunes cortical actin interaction with astral microtubules for accurate spindle orientation.
Article in Cell research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- SAPCD2 phosphorylation modulates astral microtubule stability to control spindle orientation.Communications biology · 2026Article
- Decoupling actin assembly from microtubule disassembly by TBC1D3C-mediated direct GEF-H1 activation.Life science alliance · 2025Article
- Hedgehog signaling controls astral microtubules and mitotic spindle orientation in neural progenitors and iPSCs.Frontiers in cell and developmental biology · 2025Article
- miR-31-mediated local translation at the mitotic spindle is important for early development.Development (Cambridge, England) · 2024Article
- Interplay between the plasma membrane and cell-cell adhesion maintains epithelial identity for correct polarised cell divisions.Journal of cell science · 2024Article
- PCDH1, a poor prognostic biomarker and potential target for pancreatic adenocarcinoma metastatic therapy.BMC cancer · 2023Article
- Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.Nature communications · 2023Article
- Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis.Journal of molecular cell biology · 2022Article
- Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.Nature chemical biology · 2021Article
- Article
- Chaperone-Assisted Mitotic Actin Remodeling by BAG3 and HSPB8 Involves the Deacetylase HDAC6 and Its Substrate Cortactin.International journal of molecular sciences · 2020Article
- Phase separation drives decision making in cell division.The Journal of biological chemistry · 2020Review
- Wiskott-Aldrich syndrome protein senses irradiation-induced DNA damage to coordinate the cell-protective Golgi dispersal response in human T and B lymphocytes.The Journal of allergy and clinical immunology · 2020Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oriented cell divisions are controlled by a conserved molecular cascade involving Gαi, LGN, and NuMA. Here, we show that NDP52 regulates spindle orientation via remodeling the polar cortical actin cytoskeleton. siRNA-mediated NDP52 suppression surprisingly revealed a ring-like compact subcortical F-actin architecture surrounding the spindle in prophase/prometaphase cells, which resulted in severe defects of astral microtubule growth and an aberrant spindle orientation. Remarkably, NDP52 recruited the actin assembly factor N-WASP and regulated the dynamics of the subcortical F-actin ring in mitotic cells. Mechanistically, NDP52 was found to bind to phosphatidic acid-containing vesicles, which absorbed cytoplasmic N-WASP to regulate local filamentous actin growth at the polar cortex. Our TIRFM analyses revealed that NDP52-containing vesicles anchored N-WASP and shortened the length of actin filaments in vitro. Based on these results we propose that NDP52-containing vesicles regulate cortical actin dynamics through N-WASP to accomplish a spatiotemporal regulation between astral microtubules and the actin network for proper spindle orientation and precise chromosome segregation. In this way, intracellular vesicles cooperate with microtubules and actin filaments to regulate proper mitotic progression. Since NDP52 is absent from yeast, we reason that metazoans have evolved an elaborate spindle positioning machinery to ensure accurate chromosome segregation in mitosis.
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