Evidence map›Paper›PMID 31201382›Full record

ArticleCell research2019

BubR1 phosphorylates CENP-E as a switch enabling the transition from lateral association to end-on capture of spindle microtubules.

Yuejia Huang, Lin Lin, Xing Liu, Sheng Ye, Phil Y Yao, Wenwen Wang, Fengrui Yang, Xinjiao Gao, Junying Li, Yin Zhang and 13 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 44 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

23 authors at 3 institutions in 2 countries.

Yuejia Huang *Anhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Lin Lin *National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Xing Liu *Anhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Sheng Ye *National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.ORCID http://orcid.org/0000-0002-1097-7283
Phil Y YaoDepartment of Physiology, Morehouse School of Medicine, Atlanta, GA, 30310, USA.
Wenwen WangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Fengrui YangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Xinjiao GaoAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Junying LiAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Yin ZhangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Jiancun ZhangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Zhihong YangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Xu LiuAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Zhenye YangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Jianye ZangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Maikun TengAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Zhiyong WangAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Ke RuanAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Xia DingDepartment of Physiology, Morehouse School of Medicine, Atlanta, GA, 30310, USA.
Lin LiAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China.
Don W ClevelandLudwig Institute for Cancer Research, University of California, La Jolla, CA, 92093, USA.ORCID http://orcid.org/0000-0002-1934-3682
Rongguang ZhangNational Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. rzhang@ibp.ac.cn.
Xuebiao YaoAnhui Key Laboratory for Chemical Biology & MOE Key Laboratory for Cellular Dynamics, CAS Center for Excellence in Molecular Cell Science, School of Life Sciences, Hefei National Laboratory for Physical Sciences at the Mciroscale, University of Science & Technology of China, Hefei, Anhui, 230026, China. yaoxb@ustc.edu.cn.
Morehouse School of Medicine · USChinese Academy of Sciences · CNInstitute of Biophysics · CN

Funding

Mechanisms of chromosome segregation, aneuploidy, and tumorigenesisR35GM122476 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Don W Cleveland · 2017 to 2026
$9.0M
NIGMS NIH HHS R35 GM122476
6 · The paper itself

Abstract

Error-free mitosis depends on accurate chromosome attachment to spindle microtubules, powered congression of those chromosomes, their segregation in anaphase, and assembly of a spindle midzone at mitotic exit. The centromere-associated kinesin motor CENP-E, whose binding partner is BubR1, has been implicated in congression of misaligned chromosomes and the transition from lateral kinetochore-microtubule association to end-on capture. Although previously proposed to be a pseudokinase, here we report the structure of the kinase domain of Drosophila melanogaster BubR1, revealing its folding into a conformation predicted to be catalytically active. BubR1 is shown to be a bona fide kinase whose phosphorylation of CENP-E switches it from a laterally attached microtubule motor to a plus-end microtubule tip tracker. Computational modeling is used to identify bubristatin as a selective BubR1 kinase antagonist that targets the αN1 helix of N-terminal extension and αC helix of the BubR1 kinase domain. Inhibition of CENP-E phosphorylation is shown to prevent proper microtubule capture at kinetochores and, surprisingly, proper assembly of the central spindle at mitotic exit. Thus, BubR1-mediated CENP-E phosphorylation produces a temporal switch that enables transition from lateral to end-on microtubule capture and organization of microtubules into stable midzone arrays.

Indexed as

Cell Cycle ProteinsDrosophila ProteinsProtein Serine-Threonine KinasesAnimalsCloning, MolecularDrosophila melanogasterHEK293 CellsHeLa CellsHumansMicrotubulesMitosisSf9 CellsSpindle ApparatusBUB1 protein, humanBubR1 protein, DrosophilaCell Cycle ProteinsDrosophila ProteinsProtein Serine-Threonine Kinases

Identifiers

PMID31201382
PMCPMC6796941
OpenAlexW2967567472

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.