Evidence map›Paper›PMID 31201270›Full record

ArticleThe Journal of biological chemistry2019

Design of reverse transcriptase-specific nucleosides to visualize early steps of HIV-1 replication by click labeling.

Flore De Wit, Sambasiva Rao Pillalamarri, Alba Sebastián-Martín, Akkaladevi Venkatesham, Arthur Van Aerschot, Zeger Debyser

Open access · hybridAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Flore De WitLaboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium.
Sambasiva Rao PillalamarriMedicinal Chemistry, Rega Institute for Medical Research, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium.
Alba Sebastián-MartínLaboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium.ORCID 0000-0001-7354-8964
Akkaladevi VenkateshamMedicinal Chemistry, Rega Institute for Medical Research, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium.
Arthur Van AerschotMedicinal Chemistry, Rega Institute for Medical Research, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium arthur.vanaerschot@kuleuven.be.ORCID 0000-0003-3528-8588
Zeger DebyserLaboratory for Molecular Virology and Gene Therapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, 3000 Leuven, Belgium zeger.debyser@kuleuven.be.ORCID 0000-0002-3982-1565
Rega Institute for Medical Research · BEConsejo Superior de Investigaciones Científicas · ESKU Leuven · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Only a small portion of human immunodeficiency virus type 1 (HIV-1) particles entering the host cell results in productive infection, emphasizing the importance of identifying the functional virus population. Because integration of viral DNA (vDNA) is required for productive infection, efficient vDNA detection is crucial. Here, we use click chemistry to label viruses with integrase coupled to eGFP (HIV

Indexed as

Click ChemistryVirus ReplicationAlkynesCell LineCell SurvivalDeoxyuridineDNA PrimersHIV-1HIV Reverse TranscriptaseHumansKineticsMicroscopy, ConfocalRNA, ViralAlkynesDeoxyuridineDNA PrimersHIV Reverse Transcriptasereverse transcriptase, Human immunodeficiency virus 1RNA, Viralchemical probeclick chemistryfluorescencehuman immunodeficiency virus (HIV)nucleoside/nucleotide analogpropargylated deoxynucleosidesreverse transcriptionsingle viral particle detectionviral replication

Identifiers

PMID31201270
PMCPMC6682750
OpenAlexW2949758357

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.