ArticleMicrobial cell factories2019
Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Bacterial Laccase: Recent Advances in Production, Engineering Strategies, and Diagnostic Applications.Biotechnology and bioengineering · 2026Review
- Development of a semi-endogenous PRT-based expression system for Methylotuvimicrobium alcaliphilum and its application to enhance 3-hydroxybutyric acid production from methanol.Journal of biological engineering · 2025Review
- Optimization Strategy of Expression Vectors and Regulatory Elements for Enhanced Protein Production inInternational journal of molecular sciences · 2025Review
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- Engineering a PhrC-RapC-SinR quorum sensing molecular switch for dynamic fine-tuning of menaquinone-7 synthesis in Bacillus subtilis.Microbial cell factories · 2025Article
- Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device.Microbial cell factories · 2025Article
- The Strategy and Application of Gene Attenuation in Metabolic Engineering.Microorganisms · 2025Review
- Surface display of LukF-PV onFuture microbiology · 2025Article
- Toward Antibody Production in Genome-MinimizedACS synthetic biology · 2025Article
- High-Level Expression of Sucrose Isomerase in Bacillus subtilis Through Expression Element Optimization and Fermentation Optimization.Applied biochemistry and biotechnology · 2025Article
- EngineeredACS synthetic biology · 2025Article
- ARTP/NTG Compound Mutagenesis Improved the Spinosad Production and the Insecticidal Virulence ofInternational journal of molecular sciences · 2024Article
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- The Construction of an Environmentally Friendly Super-Secreting Strain ofInternational journal of molecular sciences · 2024Article
- Secretion of the cytoplasmic and high molecular weight β-galactosidase of Paenibacillus wynnii with Bacillus subtilis.Microbial cell factories · 2024Article
- EngineeredbioRxiv : the preprint server for biology · 2024Article
- Implementation of Fluorescent-Protein-Based Quantification Analysis in L-Form Bacteria.Bioengineering (Basel, Switzerland) · 2024Article
- Efficient biosynthesis of transglutaminase inCurrent research in food science · 2024Article
- Let There Be Light: Genome Reduction EnablesACS synthetic biology · 2023Article
- Deep Learning-Assisted Design of Novel Promoters inAdvanced genetics (Hoboken, N.J.) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundBacillus subtilis is developed to be an attractive expression host to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. Chromosomal integration is a stable expression strategy while the expression level is not ideal compared with plasmid expression. Thus, to meet the requirement of protein overexpression, promoter, as one of the key elements, is important. It is necessary to obtain an ideal promoter for overproduction of foreign proteins from a single copy expression cassette.
resultsThe activity of promoter P
conclusionsA strong promoter was engineered for efficient chromosomally integrated expression of heterologous proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.