Evidence map›Paper›PMID 31200143›Full record

ArticleBiomaterials2019

Neurturin-containing laminin matrices support innervated branching epithelium from adult epithelial salispheres.

K H Vining, I M A Lombaert, V N Patel, S E Kibbey, S Pradhan-Bhatt, R L Witt, M P Hoffman

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

K H ViningMatrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 20842, USA; Medical Research Scholars Program, Office of Clinical Research Training and Medical Education, Clinical Center, NIH, Bethesda, MD, 20842, USA; University of Minnesota School of Dentistry, Minneapolis, MN 55455, USA; Current Address: John A. Paulson School of Engineering and Applied Sciences and Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, 02138. USA.
I M A LombaertMatrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 20842, USA; Current Address: Biointerfaces Institute, University of Michigan, School of Dentistry, North Campus Research Center, 2800 Plymouth Rd, Ann Arbor, MI 48104, USA.
V N PatelMatrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 20842, USA.
S E KibbeyMatrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 20842, USA.
S Pradhan-BhattDepartment of Biological Sciences, University of Delaware, Newark, DE, 19716, USA; Center for Translational Cancer Research, University of Delaware, Newark, DE, 19716, USA; Helen F. Graham Cancer Center, Christiana Care Health System, Newark, DE, 19713, USA.
R L WittDepartment of Biological Sciences, University of Delaware, Newark, DE, 19716, USA; Center for Translational Cancer Research, University of Delaware, Newark, DE, 19716, USA; Helen F. Graham Cancer Center, Christiana Care Health System, Newark, DE, 19713, USA; Otolaryngology - Head & Neck Surgery, Thomas Jefferson University, Philadelphia, PA, 19107, USA.
M P HoffmanMatrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 20842, USA. Electronic address: mhoffman@mail.nih.gov.
National Institute of Dental and Craniofacial Research · USBioSurfaces (United States) · USChristiana Care Health System · USThomas Jefferson University · USUniversity of Minnesota · US

Funding

Growth Factor/Extracellular Matrix Interactions During Branching MorphogenesisZIADE000707 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI HOFFMAN, MATTHEW · 2009 to 2023
$14.0M
Progenitor/Stem Cell Function During Salivary Gland DevelopmentZIADE000722 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI HOFFMAN, MATTHEW · 2009 to 2023
$13.7M
Growth Factor/Extracellular Matrix Interactions During Branching MorphogenesisZ01DE000707 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI HOFFMAN, MATTHEW P · 2004 to 2008
$1.8M
Anti-Inflammatory Mesenchymal Stem Cell Therapy for Dental ApplicationsK08DE025292 · NIDCR · HARVARD UNIVERSITY · PI VINING, KYLE HOLMBERG · 2016 to 2020
$635k
Neuronal and Progenitor/Stem Cell Function During Salivary Gland DevelopmentZ01DE000722 · NIDCR · NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH · PI HOFFMAN, MATTHEW · 2008 to 2008
$528k
Intramural NIH HHS Z01 DE000707Intramural NIH HHS Z01 DE000722NIDCR NIH HHS K08 DE025292
6 · The paper itself

Abstract

Cell transplantation of autologous adult biopsies, grown ex vivo as epithelial organoids or expanded as spheroids, are proposed treatments to regenerate damaged branching organs. However, it is not clear whether transplantation of adult organoids or spheroids alone is sufficient to initiate a fetal-like program of branching morphogenesis in which coordinated branching of multiple cell types including nerves, mesenchyme and blood vessels occurs. Yet this is an essential concept for the regeneration of branching organs such as lung, pancreas, and lacrimal and salivary glands. Here, we used factors identified from fetal organogenesis to maintain and expand adult murine and human epithelial salivary gland progenitors in non-adherent spheroid cultures, called salispheres. These factors stimulated critical developmental pathways, and increased expression of epithelial progenitor markers such as Keratin5, Keratin14, FGFR2b and KIT. Moreover, physical recombination of adult salispheres in a laminin-111 extracellular matrix with fetal salivary mesenchyme, containing endothelial and neuronal cells, only induced branching morphogenesis when neurturin, a neurotrophic factor, was added to the matrix. Neurturin was essential to improve neuronal survival, axon outgrowth, innervation of the salispheres, and resulted in the formation of branching structures with a proximal-distal axis that mimicked fetal branching morphogenesis, thus recapitulating organogenesis. Epithelial progenitors were also maintained, and developmental differentiation programs were initiated, showing that the fetal microenvironment provides a template for adult epithelial progenitors to initiate branching and differentiation. Further delineation of secreted and physical cues from the fetal niche will be useful to develop novel regenerative therapies that instruct adult salispheres to resume a developmental-like program in vitro and to regenerate branching organs in vivo.

Indexed as

AdultAnimalsBiocompatible MaterialsCells, CulturedEpithelial CellsEpitheliumFemaleHumansLamininMice, Inbred ICRNeurogenesisNeurturinSalivary GlandsSpheroids, CellularStem CellsTissue EngineeringBiocompatible MaterialsLamininNeurturinAdult epithelial progenitorsBioengineeringBranching morphogenesisEx vivo expansionFetal microenvironmentRegenerative medicineSalivary gland

Identifiers

PMID31200143
PMCPMC6720117
OpenAlexW2953015686

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.