ArticlePloS one2019
Identification of L-Valine-initiated-germination-active genes in Bacillus subtilis using Tn-seq.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
7 citing papers in PubMed.
- Article
- Conservation of sporulation genes and a transmembrane-containing Spo0B variant inbioRxiv : the preprint server for biology · 2025Article
- Identification and characterization of theJournal of bacteriology · 2024Article
- L-Alanine promotes anti-infectious properties of Bacillus subtilis S-2 spores via the germination receptor gerAA.Probiotics and antimicrobial proteins · 2024Article
- Mechanisms and Applications of Bacterial Sporulation and Germination in the Intestine.International journal of molecular sciences · 2022Review
- Mini Review: Bacterial Membrane Composition and Its Modulation in Response to Stress.Frontiers in molecular biosciences · 2021Review
- Survey and Validation of tRNA Modifications and Their Corresponding Genes inBiomolecules · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Bacterial endospores can survive harsh environmental conditions and long-term dormancy in the absence of nutrients, but can rapidly germinate under favorable conditions. In the present study, we employed transposon sequencing (Tn-seq) to identify genes with previously uncharacterized roles in spore germination. Identified genes that encoded spore inner membrane proteins were chosen for study of defined mutants, which exhibited delayed germination in several assays in response to varying germinants. Significantly slowed release of DPA indicated that mutants were affected in Stage I of germination. Several mutants exhibited phenotypic traits consistent with failure of a GerA germinant receptor-mediated response, while others appeared to have a more general loss of response to varied germinants. Use of a gerA-lacZ transcriptional fusion and quantitative western blotting of GerAC allowed mutants to be classified based upon normal or decreased gerA transcription and normal or reduced GerA accumulation. Fourteen genes were identified to have newly described roles within Bacillus spore germination. A more complete understanding of this process can contribute to the development of better spore decontamination procedures.
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Registered trials
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