ArticlePloS one2019
Comprehensive validation of liquid-based cytology specimens for next-generation sequencing in cancer genome analysis.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- Evaluation of DNA extraction protocols from liquid-based cytology specimens for studying cervical microbiota.PloS one · 2021Trial
- Finding More in Less: Precision Medicine for Pancreatic Cancer Using Residual Cytology Samples.Cytopathology : official journal of the British Society for Clinical Cytology · 2026Article
- Exploring alternatives to tumor tissue for BRCA1/2 next-generation sequencing testing in high-grade serous ovarian cancer: A 34-case series of malignant ascites.Cancer cytopathology · 2026Article
- Clinical utility of 'Shaken' biopsies for whole-genome sequencing.Journal of clinical pathology · 2025Article
- Analysis of endometrial liquid‑based cytology samples to detect somatic mutations and classify ovarian cancer.Oncology letters · 2025Article
- Study of Liquid-Based Cytology Using Next-Generation Sequencing as a Liquid Biopsy Application in Patients with Advanced Oncological Disease.Biomedicines · 2023Article
- Next-Generation Sequencing Analysis of Pancreatic Cancer Using Residual Liquid Cytology Specimens from Endoscopic Ultrasound-Guided Fine-Needle Biopsy: A Prospective Comparative Study with Tissue Specimens.Diagnostics (Basel, Switzerland) · 2023Article
- FFPE-Based NGS Approaches into Clinical Practice: The Limits of Glory from a Pathologist Viewpoint.Journal of personalized medicine · 2022Review
- Evaluation and diagnostic value of next-generation sequencing analysis of residual liquid-based cytology specimens of pancreatic masses.Cancer cytopathology · 2022Article
- The usefulness of liquid-based cytology for endoscopic ultrasound-guided tissue acquisition of solid pancreatic masses.Frontiers in medicine · 2022Review
- Next generation sequencing in cytology.Cytopathology : official journal of the British Society for Clinical Cytology · 2021Review
- Method for preservation of DNA stability of liquid-based cytology specimens from a lung adenocarcinoma cell line.Virchows Archiv : an international journal of pathology · 2021Article
- Molecular Evaluation of Endometrial Dedifferentiated Carcinoma, Endometrioid Carcinoma, Carcinosarcoma, and Serous Carcinoma Using a Custom-Made Small Cancer Panel.Pathology oncology research : POR · 2021Article
- Targeted next-generation sequencing assays using triplet samples of normal breast tissue, primary breast cancer, and recurrent/metastatic lesions.BMC cancer · 2020Article
- Breast cancer with an intraductal component that was proven genetically to be metastasis of contralateral breast cancer: a case report.Surgical case reports · 2020Article
- Next-generation sequencing analysis of endometrial screening liquid-based cytology specimens: a comparative study to tissue specimens.BMC medical genomics · 2020Article
- A Comparative Analysis of Diagnostic Performance and Cytomorphologic Features Between Newly Developed WellPrepJournal of cytologyArticle
- Cervical Cytology Preserves Histologically Detected Surface Epithelial Slackening, Unique to theIn vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In addition to conventional cytology, liquid-based cytology (LBC) is also used for immunocytochemistry and gene analysis. However, an appropriate method to obtain high quality DNA for next-generation sequencing (NGS) using LBC specimens remains controversial. We determined the optimal conditions for fixation with an alcohol-based fixative for LBC and DNA extraction using cultured cancer cell lines and clinical specimens. The extracted DNA was processed for NGS after the DNA quality was confirmed based on the DNA concentration and degree of degradation. The optimal conditions for cultured cells to obtain high quality DNA were to fix the cells at a density of 6 × 103 or 2 × 104 cells/mL and to use the magnetic bead-based DNA extraction method. Even after storing the fixed cells for 90 days, DNA extracted using the above and other extraction kits, including membrane-based methods, did not undergo degradation. Furthermore, 5-year-old residual LBC samples demonstrated high DNA quality that was suitable for NGS. Furthermore, a cancer genome panel analysis was successfully performed with DNA extracted from cultured cells fixed at 6 × 103 cells/mL for 90 days, and with DNA from residual LBC samples even after 1 year of storage. Residual LBC samples may be a useful source of DNA for clinical NGS to promote genome-based cancer medicine.
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