ArticlePlant biotechnology journal2020
Multiplex restriction amplicon sequencing: a novel next-generation sequencing-based marker platform for high-throughput genotyping.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Diverse reference genomes detect variants in the US winter wheat.The plant genome · 2026Article
- Current status and future perspective of genomics-assisted breeding in wheat (Breeding science · 2026Article
- Skim-sequencing for genomic selection in wheat: a comparison of marker platforms.The plant genome · 2026Article
- Integrative analysis of whole-transcriptome sequencing reveals a ceRNA regulatory network centered on P4ha1 in liver cirrhosis treated with Pien Tze Huang.EXCLI journal · 2026Article
- A low-coverage skim-sequencing and imputation pipeline for genomic selection.The plant genome · 2025Article
- Genome-wide association mapping for the identification of stripe rust resistance loci in US hard winter wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Identification of leaf rust resistance loci in hard winter wheat using genome-wide association mapping.The plant genome · 2025Article
- Streamlined whole-genome genotyping through NGS-enhanced thermal asymmetric interlaced (TAIL)-PCR.Plant communications · 2024Article
- Development and verification of a 10K liquid chip for Hainan black goat based on genotyping by pinpoint sequencing of liquid captured targets.BMC genomic data · 2024Article
- Skim exome capture genotyping in wheat.The plant genome · 2023Article
- Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii.Nature communications · 2022Article
- Development of the Wheat Practical Haplotype Graph database as a resource for genotyping data storage and genotype imputation.G3 (Bethesda, Md.) · 2022Article
- Development of the maize 5.5K loci panel for genomic prediction through genotyping by target sequencing.Frontiers in plant science · 2022Article
- Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip.Plant communications · 2021Article
- Identification of Single Nucleotide Polymorphism in Red Clover (Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To enable rapid selection of traits in marker-assisted breeding, markers must be technically simple, low-cost, high-throughput and randomly distributed in a genome. We developed such a technology, designated as Multiplex Restriction Amplicon Sequencing (MRASeq), which reduces genome complexity by polymerase chain reaction (PCR) amplification of amplicons flanked by restriction sites. The first PCR primers contain restriction site sequences at 3'-ends, preceded by 6-10 bases of specific or degenerate nucleotide sequences and then by a unique M13-tail sequence which serves as a binding site for a second PCR that adds sequencing primers and barcodes to allow sample multiplexing for sequencing. The sequences of restriction sites and adjacent nucleotides can be altered to suit different species. Physical mapping of MRASeq SNPs from a biparental population of allohexaploid wheat (Triticum aestivum L.) showed a random distribution of SNPs across the genome. MRASeq generated thousands of SNPs from a wheat biparental population and natural populations of wheat and barley (Hordeum vulgare L.). This novel, next-generation sequencing-based genotyping platform can be used for linkage mapping to screen quantitative trait loci (QTL), background selection in breeding and many other genetics and breeding applications of various species.
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