ArticleMolecular reproduction and development2019
Single cell RNA-seq in the sea urchin embryo show marked cell-type specificity in the Delta/Notch pathway.
Article in Molecular reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Metamorphosis of the purple sea urchin Heliocidaris crassispina: Single-cell transcriptomic datasets.Scientific data · 2026Article
- Article
- Embryos assist morphogenesis of others through calcium and ATP signaling mechanisms in collective teratogen resistance.Nature communications · 2024Article
- Marine Invertebrates One Cell at A Time: Insights from Single-Cell Analysis.Integrative and comparative biology · 2023Review
- Gene regulatory divergence amongst echinoderms underlies appearance of pigment cells in sea urchin development.Developmental biology · 2023Article
- Different sea urchin RAG-like genes were domesticated to carry out different functions.Frontiers in immunology · 2022Article
- Single-cell transcriptomics reveals lasting changes in the lung cellular landscape into adulthood after neonatal hyperoxic exposure.Redox biology · 2021Article
- Developmental single-cell transcriptomics in the Lytechinus variegatus sea urchin embryo.Development (Cambridge, England) · 2021Article
- Conditional gene knockdowns in sea urchins using caged morpholinos.Developmental biology · 2021Article
- Uncovering transcriptional dark matter via gene annotation independent single-cell RNA sequencing analysis.Nature communications · 2021Article
- A single cell RNA sequencing resource for early sea urchin development.Development (Cambridge, England) · 2020Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Sea urchin embryos are excellent for in vivo functional studies because of their transparency and tractability in manipulation. They are also favorites for pharmacological approaches since they develop in an aquatic environment and addition of test substances is straightforward. A concern in many pharmacological tests though is the potential for pleiotropic effects that confound the conclusions drawn from the results. Precise cellular interpretations are often not feasible because the impact of the perturbant is not known. Here we use single-cell mRNA (messenger RNA) sequencing as a metric of cell types in the embryo and to determine the selectivity of two commonly used inhibitors, one each for the Wnt and the Delta-Notch pathways, on these nascent cell types. We identified 11 distinct cell types based on mRNA profiling, and that the cell lineages affected by Wnt and Delta/Notch inhibition were distinct from each other. These data support specificity and distinct effects of these signaling pathways in the embryo and illuminate how these conserved pathways selectively regulate cell lineages at a single cell level. Overall, we conclude that single cell RNA-seq analysis in this embryo is revealing of the cell types present during development, of the changes in the gene regulatory network resulting from inhibition of various signaling pathways, and of the selectivity of these pathways in influencing developmental trajectories.
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