ArticleBone reports2019
Improved method for isolating high-quality RNA from mouse bone with RNA
Article in Bone reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- RNA-aware tissue preservation workflows for high-quality spatial transcriptomics.bioRxiv : the preprint server for biology · 2026Article
- Aging Exacerbates Binge Ethanol-Mediated Bone Toxicity in Mice.Alcohol, clinical & experimental research · 2026Article
- Acetate derived from metabolism of ethanol affects gene expression in bone and contributes to delays in chondrogenic differentiation.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Integration of phospho-signaling and transcriptomics in single cells reveals distinct Th17 cell fates.Cell reports · 2025Article
- Myeloid deficiency of heparan sulfate 6-O-endosulfatases impairs bone marrow hematopoiesis.Matrix biology : journal of the International Society for Matrix Biology · 2024Article
- The circulatory levels and bone expression of MIR21, 34a, 155 and their target genes in a section of Egyptian Population.Scientific reports · 2024Article
- Article
- Murine breast cancers disorganize the liver transcriptome in a zonated manner.Communications biology · 2023Article
- Time of day of exercise does not affect the beneficial effect of exercise on bone structure in older female rats.Frontiers in physiology · 2023Article
- Serum amyloid alpha 1-2 are not required for liver inflammation in the 4T1 murine breast cancer model.Frontiers in immunology · 2023Article
- Isolating mineralized bone and bone marrow mRNA from transiliac bone biopsies stored in a stabilizing solution: A comparative study.Bone reports · 2022Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Comparison of Different Fixation Methods for Combined Histological and Biomolecular Analysis of Fixed and Decalcified Bone Samples.Methods and protocols · 2022Article
- Nox4 expression in osteo-progenitors controls bone development in mice during early life.Communications biology · 2022Article
- Canine and murine models of osteosarcoma.Veterinary pathology · 2022Review
- A novel methodological approach to simultaneously extract high-quality total RNA and proteins from cortical and trabecular bone.Open biology · 2022Article
- Binge Ethanol Exposure in Mice Represses Expression of Genes Involved in Osteoblast Function and Induces Expression of Genes Involved in Osteoclast Differentiation Independently of Endogenous Catalase.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- An improved method for isolation of RNA from rat femur.International journal of biochemistry and molecular biology · 2022Article
- Article
- Optimization of sperm RNA processing for developmental research.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Accurate gene expression analysis of bone requires the ability to isolate RNA of good quality. Isolation of intact RNA from frozen bone tissue is problematic since RNA rapidly becomes degraded after thawing. Since we are interested in assessing gene expression from both bone marrow and mineralized bone, we aimed to develop improved simple, robust and statistically validated methods providing high-quality RNA from both mouse femur shaft and femur marrow. RNA integrity was quantified by the RNA Integrity Number (RIN) measured on a TapeStation. While the RNA stabilization reagent RNA
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Registered trials
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