Evidence map›Paper›PMID 31197896›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2019

Immunogold FIB-SEM: Combining Volumetric Ultrastructure Visualization with 3D Biomolecular Analysis to Dissect Cell-Environment Interactions.

Sahana Gopal, Ciro Chiappini, James P K Armstrong, Qu Chen, Andrea Serio, Chia-Chen Hsu, Christoph Meinert, Travis J Klein, Dietmar W Hutmacher, Stephen Rothery and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Nanoscale advances · 2023
    Article
  5. Article
  6. Article
  7. Volume electron microscopy: analyzing the lung.Histochemistry and cell biology · 2021
    Review
  8. Article
  9. Article
  10. Quantitative Particle Uptake by Cells as Analyzed by Different Methods.Angewandte Chemie (International ed. in English) · 2020
    Review
  11. High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Sahana GopalDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Ciro ChiappiniDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
James P K ArmstrongDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Qu ChenDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Andrea SerioDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Chia-Chen HsuDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID https://orcid.org/0000-0003-4408-8297
Christoph MeinertInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
Travis J KleinInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
Dietmar W HutmacherInstitute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, 4059, Australia.
Stephen RotheryFacility for Light Microscopy, Imperial College London, London, SW7 2AZ, UK.
Molly M StevensDepartment of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID https://orcid.org/0000-0002-6688-5126
NIHR Imperial Biomedical Research Centre · GBAustralian Research Council · AUKing's College London · GBImperial College London · GBQueensland University of Technology · AU

Funding

Arthritis Research UK 21138European Research Council 616417Medical Research Council MC_PC_16048Medical Research Council MR/R015651/1Medical Research Council MR/S00551X/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/R001006/1Versus Arthritis 21138Wellcome Trust 098411Wellcome Trust 098411/Z/12/Z
6 · The paper itself

Abstract

Volumetric imaging techniques capable of correlating structural and functional information with nanoscale resolution are necessary to broaden the insight into cellular processes within complex biological systems. The recent emergence of focused ion beam scanning electron microscopy (FIB-SEM) has provided unparalleled insight through the volumetric investigation of ultrastructure; however, it does not provide biomolecular information at equivalent resolution. Here, immunogold FIB-SEM, which combines antigen labeling with in situ FIB-SEM imaging, is developed in order to spatially map ultrastructural and biomolecular information simultaneously. This method is applied to investigate two different cell-material systems: the localization of histone epigenetic modifications in neural stem cells cultured on microstructured substrates and the distribution of nuclear pore complexes in myoblasts differentiated on a soft hydrogel surface. Immunogold FIB-SEM offers the potential for broad applicability to correlate structure and function with nanoscale resolution when addressing questions across cell biology, biomaterials, and regenerative medicine.

Indexed as

Cell DifferentiationDimethylpolysiloxanesEpigenesis, GeneticHumansHydrogelsImaging, Three-DimensionalMicroscopy, Electron, ScanningMyoblastsNeural Stem CellsNuclear PorebaysilonDimethylpolysiloxanesHydrogels3D reconstructionelectron microscopyFIB-SEMimage analysisimmunogold

Identifiers

PMID31197896
PMCPMC6778054
OpenAlexW2949107109

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.