Evidence map›Paper›PMID 31197538›Full record

ArticleMikrochimica acta2019

Amplified fluorescence imaging of HER2 dimerization on cancer cells by using a co-localization triggered DNA nanoassembly.

Tiantian Yang, Lulu Xu, Shengchun Liu, Yifan Shen, Lizhen Huang, Lutan Zhang, Shijia Ding, Wei Cheng

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. EGFR Expression in HER2-Driven Breast Cancer Cells.International journal of molecular sciences · 2020
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tiantian YangThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Lulu XuThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Shengchun LiuDepartment of Endocrine and Breast Surgery, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Yifan ShenThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Lizhen HuangThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Lutan ZhangThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China.
Shijia DingKey Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China. dingshijia@cqmu.edu.cn.
Wei ChengThe Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. chengwei@hospital.cqmu.edu.cn.ORCID 0000-0002-1921-9761

Funding

the National Natural Science Foundation of China 81572080the National Natural Science Foundation of China 81873972the National Natural Science Foundation of China 81873980the Training Program for Advanced Young Medical Personnel of Chongqing 2017HBRC003
6 · The paper itself

Abstract

Convenient and sensitive detection of human epidermal growth factor receptor 2 (HER2) dimerization is highly desirable for molecule subtyping and guiding personalized HER2 targeted therapy of breast cancer. A colocalization-triggered DNA nanoassembly (CtDNA) strategy was developed for amplified imaging of HER2 dimerization. It exploits (a) the advantage of the specificity of aptamer proximity hybridization, and (b) the high sensitivity of hairpin-free nonlinear HCR. The mechanism of step-by-step hairpin-free nonlinear HCR for DNA dendritic nanoassembly was studied by native polyacrylamide gel electrophoresis, atomic force microscopy and fluorometry. The results revealed a high specificity, sensitivity, and excellent controllability of the DNA dendritic nanoassembly. The method was used to identify HER2 homodimers and HER2/HER3 heterodimers in various breast cancer cell lines using fluorescence microscopy. It was then extended to image and quantitatively evaluate HER2 homodimers in clinical formalin-fixed paraffin-embedded breast cancer tissue specimens. This revealed its remarkable accuracy and practicality for clinical diagnostics. Graphical abstract Schematic presentation of amplified imaging of human epidermal growth factor receptor 2 (HER2) dimerization on cancer cell surfaces by using a co-localization triggered DNA nanoassembly (CtDNA).

Indexed as

Protein MultimerizationAptamers, NucleotideCell Line, TumorDendrimersDNAErb-b2 Receptor Tyrosine KinasesFluoresceinsFluorescent DyesHumansMicroscopy, FluorescenceNanostructuresNucleic Acid HybridizationProof of Concept Study4-carboxyfluoresceinAptamers, NucleotideDendrimersDNAERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesFluoresceinsFluorescent DyesAptamerBreast cancerCancer diagnosticsDNA nanoassemblyFluorescent imagingFormalin-fixed paraffin-embedded tissueHairpin-free nonlinear HCRHER2 dimerizationProximity hybridization

Identifiers

PMID31197538

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.