Evidence map›Paper›PMID 31197469›Full record

ArticleMikrochimica acta2019

Carbon nanotube-based lateral flow immunoassay for ultrasensitive detection of proteins: application to the determination of IgG.

Wanwei Qiu, Kwaku Baryeh, Sunitha Takalkar, Wei Chen, Guodong Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Nanomaterial and interface advances in immunoassay biosensors.The journal of physical chemistry. C, Nanomaterials and interfaces · 2022
    Article
  5. Review
  6. Review
  7. Review
  8. Magnetic Lateral Flow Immunoassays.Diagnostics (Basel, Switzerland) · 2020
    Review
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 3 countries.

Wanwei QiuInstitute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China.
Kwaku BaryehDepartment of Chemistry and Biochemistry, North Dakota State University, Fargo, ND, 58105, USA.
Sunitha TakalkarInstitute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China.
Wei ChenDepartment of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. chenweishnu@hfut.edu.cn.
Guodong LiuInstitute of Biomedical and Health Science, School of Life and Health Science, Anhui Science and Technology University, Fengyang, 233100, Anhui, China. guodong.liu@ndsu.edu.ORCID 0000-0003-1063-208X
Anhui University of Science and Technology · CNCouncil for Scientific and Industrial Research · GHHefei University of Technology · CNSt. Jude Children's Research Hospital · US

Funding

Targeting CLEC-2/podoplanin as a therapeutic strategy for pancreatic cancerP20GM109024 · NIGMS · NORTH DAKOTA STATE UNIVERSITY · PI Megan Orr · 2016 to 2026
$20.5M
NIGMS NIH HHS P20 GM109024the National Natural Science Foundation of Anhui Province 1908085MB54
6 · The paper itself

Abstract

Authors report on a carbon nanotube (CNT)-based lateral flow immunoassay (LFI) for ultrasensitive detection of proteins. Shortened multiwalled CNTs were used as a colored (black) tag. The detection antibody was covalently immobilized on the CNT surface via diimide-activated conjugation between the carboxyl groups on the CNT surface and amino groups of antibodies. The assay involved the capture of target protein in a sandwich-type format between an immobilized capture antibody on the test zone of LFI and a CNT-labelled detection antibody. CNTs were thus captured on the test zone of the LFI and gave a black colored line to enable visual detection of protein. Quantitative results were obtained by reading the test line intensities with a portable strip reader. Rabbit IgG was used as a model target to demonstrate the proof-of-concept. Combining the advantages of lateral flow assay with the unique physical properties of CNT (color, high aspect-to-size ratio and ease of surface modification), the optimized LFI can detect of 1.3 pg mL

Indexed as

AnimalsAntibodies, ImmobilizedHumansImmunoassayImmunoglobulin GLimit of DetectionNanotubes, CarbonProof of Concept StudyRabbitsAntibodies, ImmobilizedImmunoglobulin GNanotubes, CarbonCarbon nanotubeImmunoassayLateral flow assayProtein analysisRabbit IgG

Identifiers

PMID31197469
OpenAlexW2949104931

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.