Evidence map›Paper›PMID 31197357›Full record

ArticlePathogens and disease2019

A primary study on genes with selected mutations by in vitro passage of Chlamydia muridarum strains.

Zhou Zhou, Na Liu, Yingzi Wang, Arthur Wirekoh Emmanuel, Xiaoxing You, Jiulin Liu, Zhongyu Li, Yimou Wu, Guangming Zhong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pathogens and disease, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Frontiers in cellular and infection microbiology · 2026
    Review
  2. Complete deletion of theInfection and immunity · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Zhou ZhouInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Na LiuInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Yingzi WangInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Arthur Wirekoh EmmanuelInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Xiaoxing YouInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Jiulin LiuOutstanding Physician Class in Grade 2016, Medical College, University of South China, Hengyang 421001, Hunan, China.
Zhongyu LiInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Yimou WuInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
Guangming ZhongInstitute of Pathogenic Biology, Medical College, University of South China, Hengyang 421001, Hunan, China.
University of South China · CNThe University of Texas Health Science Center at San Antonio · US

Funding

Chlamydial plasmid-dependent pathogenesisR01AI121989 · NIAID · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI ZHONG, GUANGMING · 2016 to 2019
$1.9M
NIAID NIH HHS R01 AI121989
6 · The paper itself

Abstract

objectiveThis study is to investigate the functions of newly discovered genes in Chlamydia muridarum (C. muridarum) strains with single gene differences.

methodsUsing whole genome sequencing and plaque formation assays, C. muridarum parental and passaging strains were established, and the isogenic clones expressing certain genotypes were isolated. Strains with single gene differences were obtained. Based on prediction, the valuable strains with single gene differences of tc0412, tc0668 or tc0237 were subjected to the in vitro and in vivo experiments for biological characterization and virulence analysis.

resultsInsertional -472840T mutation of the tc0412 gene (T28T/B3 type) matching with the nonmutant tc0668 gene and tc0237 gene with point mutations G797659T (Q117E) might slow the growth of Chlamydia due to the lack of a plasmid. The nonmutant tc0668 in the strain might induce a high incidence of hydrosalpinx in mice, while tc0668 with a G797659T point mutation was significantly attenuated. Compared with the nonmutant tc0237, the strains containing mutant tc0237 were characterized by reduced centrifugation dependence during infection.

conclusionThe identification and characterization of these genes might contribute to the comprehensive understanding of the pathogenic mechanism of Chlamydia.

Indexed as

Genes, BacterialGenetic VariationMutationAnimalsBacterial LoadChlamydia InfectionsChlamydia muridarumDisease Models, AnimalFemaleGenotypeHeLa CellsHumansMice, Inbred C3HReproductive Tract InfectionsSerial PassageVaginabiological characteristicsChlamydia muridarum (C. muridarum)pathogenic mechanismurogenital infection

Identifiers

PMID31197357
PMCPMC7962888
OpenAlexW2951254003

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.