ArticleNature communications2019
Coherent diffractive imaging of microtubules using an X-ray laser.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Biological applications at the AQUA beamline of the EuPRAXIA@SPARC_LAB free electron laser.European biophysics journal : EBJ · 2026Article
- High Precision Antibody-Free Microtubule Labeling for Expansion Microscopy.Bio-protocol · 2025Article
- Scalable 3D reconstruction for X-ray single particle imaging with online machine learning.Nature communications · 2025Article
- Serial coherent diffraction imaging of dynamic samples based on inter-frame continuity.Light, science & applications · 2025Article
- Comprehensive RNA-seq analysis of benign prostatic hyperplasia (BPH) in rats exposed to testosterone and estradiol.Scientific reports · 2025Article
- Ultra-Thin Flexible Encapsulating Materials for Soft Bio-Integrated Electronics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Neurotransmitter uptake of synaptic vesicles studied by X-ray diffraction.European biophysics journal : EBJ · 2022Article
- Low-dose shift- and rotation-invariant diffraction recognition imaging.Scientific reports · 2022Article
- MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography.Nature communications · 2021Article
- Holography and Coherent Diffraction Imaging with Low-(30-250 eV) and High-(80-300 keV) Energy Electrons: History, Principles, and Recent Trends.Materials (Basel, Switzerland) · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
33 authors at 6 institutions in 5 countries.
Funding
Abstract
X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.