ArticleMicrobial cell factories2019
Deciphering the crucial roles of transcriptional regulator GadR on gamma-aminobutyric acid production and acid resistance in Lactobacillus brevis.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review.International journal of molecular sciences · 2020Pooled it
- Comparative evaluation of the gastroprotective effects of lactic acid bacteria strains against ethanol/HCl-induced gastric injury.Food science of animal resources · 2026Article
- Lactic Acid Bacteria-Derived γ-Aminobutyric Acid: From Targeted Screening and Biosynthesis to Functional Food Applications and Health Benefits.Foods (Basel, Switzerland) · 2026Review
- Engineering Strategies and Operations of Frozen Yogurt Formulated With GABA-Enriched Yogurt: A Review.Journal of food science · 2026Review
- GABA-Producing Bacteria as Potential Psychobiotics in Gut-Brain Axis Regulation.International journal of molecular sciences · 2026Review
- Effect of Glutamate Concentration and Atmosphere of Incubation on the Production of ɣ-Aminobutyric Acid inMicroorganisms · 2026Article
- Influence of Peptide-Rich Nitrogen Sources on GAD System Activation and GABA Production inInternational journal of molecular sciences · 2025Article
- Development and Genomic Evaluation of a Novel Functional Fermented Milk Formulated withFoods (Basel, Switzerland) · 2025Article
- A plasmid-borne gadRCB operon contributes to acid tolerance in Lactiplantibacillus plantarum ZR79.Applied microbiology and biotechnology · 2025Article
- A Novel GABA-ProducingBiomolecules · 2025Article
- ProbioticCurrent research in food science · 2025Article
- GlnR positively affects the acid resistance ofFrontiers in microbiology · 2025Article
- Contributions of Gamma-Aminobutyric Acid (GABA) Produced by Lactic Acid Bacteria on Food Quality and Human Health: Current Applications and Future Prospects.Foods (Basel, Switzerland) · 2024Review
- Effect of Goji Berry extract on cell viability ofFood science and biotechnology · 2024Article
- GABA synthesizing lactic acid bacteria and genomic analysis of3 Biotech · 2024Article
- Comprehensive characterization of γ-aminobutyric acid (GABA) production byFrontiers in microbiology · 2024Article
- Natural diversity of lactococci in γ-aminobutyric acid (GABA) production and genetic and phenotypic determinants.Microbial cell factories · 2023Article
- Expression and Transformation Characteristics of a Novel Glutamic Acid Decarboxylase LcGAD10s and Its Application on Sufu Processing.Foods (Basel, Switzerland) · 2023Article
- Development of a workflow for the selection, identification and optimization of lactic acid bacteria with high γ-aminobutyric acid production.Scientific reports · 2023Article
- Optimization of strains for fermentation of kiwifruit juice and effects of mono- and mixed culture fermentation on its sensory and aroma profiles.Food chemistry: X · 2023Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIn lactic acid bacteria (LAB), acid stress leads to decreases of cell vitality and fermentation yield. Glutamate decarboxylase (GAD) system is regarded as one of the essential acid-resistance mechanisms in LAB. However, the regulation of GAD system is not well identified in the genus Lactobacillus. Although potential transcriptional regulator gene located upstream of GAD system genes was found in several Lactobacillus species, such as Lactobacillus (L.) brevis, the contribution of the regulator to acid resistance of the genus Lactobacillus has not been experimentally determined.
resultsThe potential transcriptional regulator gene gadR was disrupted by homologous recombination in L. brevis ATCC 367, leading to the decreased expression of gadC and gadB. The inactivation of GadR completely eliminated γ-aminobutyric acid (GABA) production and decreased the glutamate-dependent acid resistance. Moreover, expression of gadC and gadB in the presence of glutamate was increased and glutamate also stimulated the expression of gadR. In addition, L. brevis D17, a strain screened from acidic fermented grains of Chinese liquor production, had much higher expression level of gadR than the typical strain L. brevis ATCC 367. Under the pH-controlled and mixed-feed fermentation, L. brevis D17 achieved a titer of 177.74 g/L and a productivity of 4.94 g/L/h of GABA within 36 h. However, the L. brevis ATCC 367 only achieved a titer of 6.44 g/L and 0.18 g/L/h of GABA although the same fermentation control approach was employed.
conclusionsGadR is a positive transcriptional regulator controlling GABA conversion and acid resistance in L. brevis. L. brevis strains with hyper-expressing of gadR are excellent candidates for GABA production in industrial scale.
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