Evidence map›Paper›PMID 31195428›Full record

ArticleCurrent protocols in mouse biology2019

High Resolution Imaging of Mouse Embryos and Neonates with X-Ray Micro-Computed Tomography.

Chih-Wei Hsu, Sowmya Kalaga, Uchechukwu Akoma, Tara L Rasmussen, Audrey E Christiansen, Mary E Dickinson

Abstract read
In one paragraph

Article in Current protocols in mouse biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Three-dimensional microCT imaging of mouse heart development from early post-implantation to late fetal stages.Mammalian genome : official journal of the International Mammalian Genome Society · 2023
    Review
  7. Article
  8. Article
  9. Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022
    Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chih-Wei HsuDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.
Sowmya KalagaDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.
Uchechukwu AkomaDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.
Tara L RasmussenDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.
Audrey E ChristiansenDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.
Mary E DickinsonDepartment of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas.

Funding

UM1HG006348: Cas9 Genome Integrity Supplemental ProposalUM1HG006348 · NHGRI · BAYLOR COLLEGE OF MEDICINE · PI Jason D. Heaney, Chih-Wei Logan Hsu · 2016 to 2026
$47.5M
National Institutes of Health (NIH) UM1 HG006348NHGRI NIH HHS UM1 HG006348
6 · The paper itself

Abstract

Iodine-contrast micro-computed tomography (microCT) 3D imaging provides a non-destructive and high-throughput platform for studying mouse embryo and neonate development. Here we provide protocols on preparing mouse embryos and neonates between embryonic day 8.5 (E8.5) to postnatal day 4 (P4) for iodine-contrast microCT imaging. With the implementation of the STABILITY method to create a polymer-tissue hybrid structure, we have demonstrated that not only is soft tissue shrinkage minimized but also the minimum required time for soft tissue staining with iodine is decreased, especially for E18.5 to P4 samples. In addition, we also provide a protocol on using commercially available X-CLARITY

Indexed as

MiceAnimalsAnimals, NewbornContrast MediaEmbryo, MammalianIodine CompoundsX-Ray MicrotomographyContrast MediaIodine Compoundsiodine potassium iodideiodine contrastingmicroCTSTABILITYX-CLARITY

Identifiers

PMID31195428
PMCPMC6594408

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.